| 百合病毒检测技术研究进展 |
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| 引用本文： | 贾慧,王进忠,陈忠斌,王升启,董金皋,赵祥云,张民照.百合病毒检测技术研究进展[J].北京农学院学报,2004,19(4):73-76. |
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| 作者姓名： | 贾慧 王进忠 陈忠斌 王升启 董金皋 赵祥云 张民照 |
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| 作者单位： | 河北农业大学植物保护学院,保定,071001;北京农学院植物科学技术系,北京,102206;军事医学科学院放射医学研究所,北京100850 |
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| 摘 要： | 笔者对百合病毒的检测技术作了综合评述,主要有植株直接观测法、指示植物接种鉴定法、电镜技术、血清学检测和分子生物学检测技术等,综合应用各项技术使检测更加灵敏、可靠。目前,常用的检测以血清学技术的ELISA为主、分子生物学技术的RT PCR为主。基因芯片检测技术是近几年发展起来高效的检测手段并在植物病毒检测上有广泛的应用前景。
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| 关 键 词： | 百合 病毒 检测 |
| 文章编号： | 1002-3186(2004)04-0073-04 |
| 修稿时间： | 2004年4月8日 |
Advances in Detection Techniques of Lily virus |
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| JIA Hui,WANG Jin-zhong,CHEN Zhong-bin,WANG Sheng-qi,DONG Jin-gao,ZHAO Xiang-you,ZHANG Ming-zhao.Advances in Detection Techniques of Lily virus[J].Journal of Beijing Agricultural College,2004,19(4):73-76. |
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| Authors: | JIA Hui WANG Jin-zhong CHEN Zhong-bin WANG Sheng-qi DONG Jin-gao ZHAO Xiang-you ZHANG Ming-zhao |
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| Institution: | JIA Hui~3,WANG Jin-zhong~1*,CHEN Zhong-bin~2,WANG Sheng-qi~2,DONG Jin-gao~3,ZHAO Xiang-you~1,ZHANG Ming-zhao~1 |
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| Abstract: | The advances in detection techniques of Lily virus were introduced. The detection techniques mainly include the plant directly watching, a method for sensitive indicator plants, scan electron microscope ,a serological detection method, and molecular biology technique. Integrated application of all kinds of detecting means makes it more sensitive and reliable. At present, serological detection is mainly based on ELISA and molecular biology technique on RT-PCR. The detecting technology of gene chip is an effective detecting method developed in recent years as well it has extensive application prospect in Plant virus detecting. |
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| Keywords: | lily virus detection |
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