Microsatellite diversity and effective population size in a germplasm bank of <Emphasis Type="Italic">Hymenaea courbaril</Emphasis> var. <Emphasis Type="Italic">stilbocarpa</Emphasis> (Leguminosae), an endangered tropical tree: recommendations for conservation |
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Authors: | Juliana Massimino Feres Marcela Corbo Guidugli Moacyr Antonio Mestriner Alexandre Magno Sebbenn Ana Yamaguishi Ciampi Ana Lilia Alzate-Marin |
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Institution: | 1.Plant Genetic Laboratory, Department of Genetics, FMRP,University of S?o Paulo (USP),Ribeir?o Preto,Brazil;2.Graduate Program in Genetics, Department of Genetics, FMRP,University of S?o Paulo (USP),Ribeir?o Preto,Brazil;3.Graduate Program in Comparative Biology, Department of Biology, FFCLRP,University of S?o Paulo (USP),Ribeir?o Preto,Brazil;4.Experimental Station Tupi, Forest Institute of S?o Paulo,Piracicaba,Brazil;5.Embrapa Cenargen, PqEB,Brasília,Brazil |
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Abstract: | Deforestation in southeast Brazil has led to the extinction of Hymenaea courbaril var. stilbocarpa and ex situ conservation has been established. In this study, the levels of genetic diversity and the effective population size of H. courbaril in a germplasm bank were investigated using six nuclear microsatellite loci. A total of 79 and 91 alleles were found in 65
seed-trees and their 176 offspring, respectively. Offspring have a higher average number of alleles per locus (A = 15.2) than seed-trees (A = 13.2), but lower observed heterozygosity (offspring: H
o = 0.566; seed-trees: H
o = 0.607). The estimate of outcrossing rate shows that the study population is perfectly outcrossed (t
m
= 0.978, P > 0.05). Significant deviations from random mating were detected through mating among relatives and correlated matings. The
average variance in effective population size for each family was 2.63, with a total effective population size retained in
the bank of 170.1. These results confirm that the preserved population of H. courbaril retains substantial genetic variability.
Moacyr Antonio Mestriner and Ana Lilia Alzate-Marin—CEEFLORUSP Members. |
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Keywords: | BG/USP/RP Ex situ conservation Germplasm bank Hymenaea courbaril Jatobá Molecular marker Tropical tree |
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