乙醇溶剂与超临界CO_2相结合提取高纯度卵黄磷脂的研究

采用乙醇溶剂首先提取蛋黄粉中的卵黄油 ,然后用超临界 CO2 萃取方法脱除卵黄油中的中性脂肪 ,获得高纯度的卵黄磷脂。考察了工艺参数对提取效果的影响。试验表明 :在乙醇溶剂提取卵黄油阶段 ,乙醇浓度是影响卵黄油提取率的最主要因素 ,温度是影响卵黄磷脂提取率的最主要因素 ;在超临界 CO2 萃取阶段 ,磷脂的溶解度随萃取温度的升高而降低 ,中性脂质在 5 5℃时溶解度最大。采用此工艺 ,卵黄磷脂得率为 17.6 6 % ,纯度 94.41% ,且不含胆固醇。

High Purity Phospholipids Extraction From Egg Yolk Using Ethanol and SC-CO_2

A two-step process using ethanol and supercritical carbon dioxide(SC-CO2) to extract phospholipids from egg yolk was developed. The processing parameters on the separating effects were investigated. Experimental results show: at the ethanol extracting stage, ethanol concentration is the major factor affecting the extracting rate of total yolk lipids, temperature is the major factor affecting the extracting rate of phospholipids. At the SC-CO2 extracting stage, the solubility of phospholipids increases with temperature, and the solubility of neutral lipids reaches the highest at 55℃. The two-step process has good separating effect, phospholipids is of high yield(17.66%) and high purity (94.41%), and the yolk protein has little denaturation.