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花粉管通道介导玉米总DNA导入小麦D1代的农艺性状研究
引用本文:胡文明,徐翠莲,王岳光.花粉管通道介导玉米总DNA导入小麦D1代的农艺性状研究[J].湖北农业科学,2007,46(6):867-869.
作者姓名:胡文明  徐翠莲  王岳光
作者单位:1. 塔里木大学植物科技学院,新疆,阿拉尔,843300;青岛农业大学,山东,青岛,266109
2. 塔里木大学植物科技学院,新疆,阿拉尔,843300
3. 青岛农业大学,山东,青岛,266109
基金项目:山东省三零工程项目 , 青岛农业大学博士基金
摘    要:采用花粉管通道法介导玉米总DNA导入4个小麦品种(系)"9411"、"955159"、"96266"和鲁麦21中,并对转基因D1代的农艺性状进行了分析.研究发现,与对照相比,鲁麦21和"96266"的转基因D1代株高、穗长的差异达极显著水平;"9411"、"955159"、"96266"的转基因D1代的旗叶叶长和叶面积均达极显著水平;在"9411"的转基因D1代中,出现无芒变异的植株占20%左右;在"9411"的转基因D1代中,也发现了穗型与抗病性同时发生变异的植株.

关 键 词:小麦  花粉管通道法  玉米总DNA  转基因D1代
文章编号:0439-8114(2007)06-0867-03
收稿时间:2007-08-26
修稿时间:2007年8月26日

Characteristics of the Transgenic Wheat D1 Generation from Total Corn DNA through Pollen Tube Pathway
HU Wen-ming,XU Cui-lian,WANG Yue-guang.Characteristics of the Transgenic Wheat D1 Generation from Total Corn DNA through Pollen Tube Pathway[J].Hubei Agricultural Sciences,2007,46(6):867-869.
Authors:HU Wen-ming  XU Cui-lian  WANG Yue-guang
Abstract:The total DNA of corn was introduced into wheat "9411","955159","96266",Lumai 21 through pollen tube pathway.Characteristics of the transgenic wheat D1 generation was studied.The results were as follows: compared with the control group,the transgenic D1 generation of Lumai 21 and "96266" varied extremely in plant height and ear length respectively;the transgenic D1 generation of "9411","955159","96266" varied extremely in length and square of the flag leaf;The variation rate was about 20% of beardless ear in transgenic D1 generation of "9411";The variation of ear shape and disease resistance was found in the same plant in transgene D1 generation of "9411".
Keywords:wheat  pollen tube pathway  total corn DNA  the transgenic D1 generation
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