基于三级营养传递Cry1Ac蛋白对普通草蛉酶活力及生长发育的影响

【目的】研究Cry1Ac蛋白通过棉花、棉蚜的传递对普通草蛉酶活性及生长发育的影响,为转Bt基因棉花的安全性评价提供参考和依据。【方法】采用ELISA方法,检测Bt棉、取食Bt棉的棉蚜和捕食棉蚜普通草蛉体内Bt杀虫蛋白含量;通过酶活力测定普通草蛉幼虫取食Bt棉花上棉蚜后其体内的类胰蛋白酶、类胰凝乳蛋白酶和氨肽酶的酶活性;室内生物测定普通草蛉取食Bt棉花上的棉蚜后对其生长发育的影响。【结果】Cry1Ac杀虫蛋白在棉叶中的表达量为788.76 ng/g,远高于棉蚜体内的2.35 ng/g,取食Bt棉花上棉蚜的普通草蛉3龄幼虫体内并未检测到Bt杀虫蛋白;通过三级营养传递的Cry1Ac蛋白对普通草蛉酶活力影响甚微。【结论】棉蚜取食Bt棉花后体内仅能累计微量Cry1Ac蛋白;普通草蛉取食Bt棉花上的棉蚜后,对其体内三种酶活性和生长发育均没有负面影响。

Effects of Cry1Ac Protein on Enzyme Activity and Development of Chrysoper carnea Based on Tertiary Nutrition Chain

【Objective】 To study the effect of Cry1Ac protein transfer through cotton and cotton aphid on the enzyme activity and growth and development of Chrysoper carnea, and to provide reference and basis for the safety evaluation of transgenic BT cotton.【Method】 Testing the Bt protein content of Bt cotton, Aphis gossypii fed on Bt cotton and the Chrysoper carnea reared on Aphis gossypii by ELISA method; measuring the Trypsin-like, Chymotrypsin-like and Aminopeptidase N of Chrysoper carnea reared on Aphis gossypii on Bt cotton by enzyme activity assay, researching on growth and development of theChrysoper carnea reared on Aphis gossypii on Bt cotton by bioassay.【Result】 Cry1Ac protein content in cotton leaves was 788.76 ng/g, more than the expression in Aphis gossypii which was 2.35 ng/g , and BT insecticidal protein was not detected in the 3rd instar larvae feeding on BT cotton. Cry1Ac protein transmitted through tertiary nutrition chain had little effect on the enzyme activity of Chrysoper carnea. 【Conclusion】The accumulation of Cry1Ac protein in Aphis gossypii after fed on BT cotton was very small, while that of cotton aphid on BT cotton had no negative effect on the activity of three enzymes and the growth and development of Chrysoper carnea.