甘蓝型油菜固醇载体蛋白2基因BnSCP-2的克隆与表达

固醇载体蛋白2(SCP-2)是一类小分子碱性蛋白,参与细胞内脂质运输、脂肪酸代谢以及过氧化物酶体β-氧化等过程。利用同源序列设计引物进行PCR扩增,克隆得到甘蓝型油菜固醇载体蛋白2基因的c DNA序列,命名为BnSCP-2。BnSCP-2的开放阅读框长372bp,编码一个由123个氨基酸残基组成的蛋白质,预测的蛋白质相对分子质量为13.52kD,等电点为9.35。SCP-2蛋白氨基酸序列非常保守,系统进化分析表明BnSCP-2与拟南芥SCP-2同源性最高。荧光实时定量PCR结果表明BnSCP-2在检测的各个组织均有表达,其中花和花蕾中的表达量最高;赤霉素、细胞分裂素和脱落酸等可显著诱导幼苗中该基因的表达,推测该基因可能参与调控幼苗生长发育。

Cloning and expression of sterol carrier protein 2 gene from Brasscia napus

Sterol carrier protein 2 (SCP-2) is a small, basic protein involved in intracellular lipid circulation, fatty acid metabolism and peroxisomal β-oxidation. Based on the homologous sequence, a cDNA fragment of SCP-2 gene was cloned in Brassica napus, and designated as BnSCP-2. The complete open reading frame of BnSCP-2 is 372bp in length, encoding 123 amino acid with a predicted molecular mass of 13.52kD and a pI of 9.35. The amino acids of SCP-2 protein were highly conserved. Phylogenetic analysis revealed that deduced BnSCP-2 protein was close to SCP-2 protein in Arabidopsis. Realtime RT-PCR showed that BnSCP-2 was expressed in all tissues tested, with the highest expression in flowers and flower buds. Its expression in seedlings was significantly up-regulated by GA3, 6-BA and ABA treatments, suggesting that BnSCP-2 may be involved in growth regulation and young seedling development.