| 羊口疮病毒的分离与鉴定 |
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| 引用本文： | 庞方圆,高日明,李旭东,闫聪,李浩,王艳杰,张七斤.羊口疮病毒的分离与鉴定[J].中国畜牧兽医,2015,42(2):467-471. |
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| 作者姓名： | 庞方圆 高日明 李旭东 闫聪 李浩 王艳杰 张七斤 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院, 呼和浩特 010018;2. 金宇保灵生物药品有限公司, 呼和浩特 010030;3. 兽用疫苗国家工程实验室, 呼和浩特 010010 |
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| 基金项目： | 兽用疫苗国家工程实验室项目 |
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| 摘 要： | 本试验利用犊牛睾丸细胞、羔羊睾丸细胞、MDBK、BHK-21细胞对采自内蒙古赤峰的疑似羊口疮发病羊群的唇部痂皮组织进行接种传代,分离出1株病毒,通过透射电镜负染观察和PCR检测对该分离株进行鉴定。参照GenBank中羊口疮病毒(Orf virus,ORFV)ORF059(F1L)基因的核苷酸序列,设计并合成1对特异性引物,成功地对分离株的F1L基因进行克隆、测序,并与多株参考毒株进行了同源性比对分析,结果显示经透射电镜负染观察到典型的副痘病毒粒子,与参考毒株序列相比均具有较高的同源性,均为96%以上,结果表明该分离株为ORFV,命名为OV/nm-hd。
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| 关 键 词： | 羊口疮病毒 分离鉴定 F1L基因 序列分析 |
| 收稿时间： | 2014-07-28 |
Isolation and Identification of Orf Virus |
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| PANG Fang-yuan,GAO Ri-ming,LI Xu-dong,YAN Cong,LI Hao,WANG Yan-jie,ZHANG Qi-jin.Isolation and Identification of Orf Virus[J].China Animal Husbandry & Veterinary Medicine,2015,42(2):467-471. |
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| Authors: | PANG Fang-yuan GAO Ri-ming LI Xu-dong YAN Cong LI Hao WANG Yan-jie ZHANG Qi-jin |
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| Institution: | 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2. The Spirit Jinyu Biological Pharmaceutical Co., Ltd., Hohhot 010030, China;3. Veterinary Vaccines National Engineering Laboratory, Hohhot 010010, China |
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| Abstract: | One strain of virus was isolated by passage culture in bovine testis cell,lamb testis cell,MDBK cell and BHK-21 cell of lip crust tissue collected from suspected Orf sheep in Chifeng,Inner Mongolia.The isolated virus was identified through negative staining by TEM observation and PCR detection method.One pair of specific primers was designed and synthesized referring to nucleotide sequence of Orf virus (ORFV) ORF059 (F1L) gene in GenBank.F1L gene of isolated virus was successfully cloned and sequenced,the homology was analyzed with many reference strains.The result showed that typical brick-shaped virion was found by TEM observation,and the isolated strains had high homologies with reference strains,the homologies were all more than 96%,illustrating that the isolated strains was ORFV,named as OV/nm-hd. |
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| Keywords: | Orf virus isolation and identification F1L gene sequence analysis |
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