四川省部分地区山羊沙门氏菌健康带菌率及耐药性调查

为了解四川省山羊沙门氏菌带菌情况,本试验采集了四川省部分地区5个规模化山羊养殖场表观健康山羊粪便196份,经BPW预增菌、TTB选择性增菌后,采用靶向invA基因的PCR方法检测沙门氏菌的带菌率;对阳性样本进行细菌分离鉴定,随机选择25株沙门氏菌分离株测定其对15种抗菌药物的敏感性。结果显示,山羊沙门氏菌的平均带菌率为54.59%。药敏试验结果显示,分离株均对丁胺卡那敏感,而对其余14种抗菌药物呈不同程度的耐药,多重耐药菌株占88%,其中耐2~7种药物的占52%,耐9~13种药物的占36%。结果表明,四川省部分地区山羊的沙门氏菌健康带菌率较高,且多重耐药性普遍,其公共卫生意义值得进一步研究。

Prevalence and Resistance Analysis of Salmonella in Clinical Healthy Goats in Sichuan Province

To investigate the prevalence of Salmonella in clinical healthy goats in Sichuan province,a total of 196 fecal samples collected from 5 goat farms in Sichuan province were tested in this study.Each sample was pre-enriched by buffered peptone water (BPW),and then selectively enriched by tetrathionate broth base (TTB).The enrichments were detected by PCR assay targeting the invA gene to determine the presence of Salmonella.The positive samples detected by PCR were isolated and identified; 25 Salmonella isolates were randomly selected to test the antibiotic resistance against 15 antibiotics.The results showed that the positive rate of Salmonella in fecal samples was 54.59%.Antibiotic sensitivity analysis showed that all of the tested strains were sensitive to amikacin,while the strains were resistant to the other 14 antibiotics in certain degrees.The rate of multi-drug resistance was 88%,and among the 25 isolates,52% were resistant to 2 to 7 antibiotic and 36% were resistant to 9 to 13 antibiotics.The results indicated that Salmonella were prevalent among clinical healthy goats in Sichuan province,and the multi-drug resistance of the Salmonella isolates from goats were quite common,which had significant implication for public health.