| 广西北海红树林土壤放线菌的分离与鉴定 |
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| 引用本文： | 廖振林,刘菁,陈建宏,钟毓娟,蒋莲秀,陈森洲. 广西北海红树林土壤放线菌的分离与鉴定[J]. 农业科学与技术, 2010, 0(5) |
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| 作者姓名： | 廖振林 刘菁 陈建宏 钟毓娟 蒋莲秀 陈森洲 |
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| 作者单位： | 廖振林(华南农业大学食品学院生物工程系,广东广州,510642);刘菁,陈建宏,钟毓娟,蒋莲秀,陈森洲(桂林医学院微生物学与免疫学教研室,广西桂林,541004) |
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| 基金项目： | 广西科学基金项目，华南农业大学校长基金(5100-k05099).Supported by Science Foundation of Guangxi Province，South China Agricultural University Principal Foundation |
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| 摘 要： | [目的]鉴定广西北海红树林土壤放线菌的物种多样性.[方法]从广西北海红树林土壤中分离、筛选10株典型放线菌菌株,提取基因组DNA,进行16S rDNA PCR扩增与测序,并构建进化树.[结果]通过Blast比对,10株放线菌属于2个属,其中8株为链霉菌属(80%),2株为拟诺卡氏菌属(20%).[结论]广西北海红树林土壤蕴减着种类丰富的放线菌.Abstract：[Objective] The aim was to identify the species diversity of Actinomycetes from Mangrove forest in Beihai,Guangxi Province.[Method]10 strains of typical Actinomycetes were isolated from Mangrove forest soil,and the Actinomycetes genomic DNA was successful extracted.16S rDNA was amplified by PCR and sequenced by Sanger dideoxy sequencing method.[Rcsult]All the sequences were blasted in genbank,eight strains belonged to the genus of Streptomyces (80%),and two strains belonged to the genus of Nocardiopsis (20%).[Coacluslon]There are many different Actinomycetes species in Mangrove forest soil samples in Beihai,Guangxi Province.
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| 关 键 词： | 广西 北海 红树林土壤 土壤放线菌 分离与鉴定 Actinobacteria Identification of forest soil species diversity 拟诺卡氏菌属 genomic DNA 物种多样性 构建进化树 放线菌菌株 successful different Blast比对 链霉菌属 rDNA typical |
Isolation and Identification of Soil Actinobacteria from Mangrove forest in Beihai,Guangxi Province |
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| Abstract: | Mangrove soil Actinomycetas 16S rDNA Streptomyces |
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| Keywords: | 16S rDNA |
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