青蒿组织中快速有效DNA提取方法的研究

[目的]探索适合于快速有效的提取青蒿DNA的方法。[方法]以青蒿苗期幼叶、抽穗期幼穗及抽穗期老叶为材料,采用剪碎法、石英法及液氮法等3种方法提取DNA,并进行吸光度检测、琼脂糖电泳及PCR扩增以检测DNA提取效果。[结果]剪碎法提取的DNA纯度较高,适合于PCR扩增,同时节省了时间和成本。试验结果还表明,青蒿的抽穗期幼穗更适合于提取DNA。[结论]剪碎法是一种适合于提取青蒿DNA的快速有效的方法。

An Improved Method of Extracting Artemisoa abrotanum Genomic DNA

[Objective] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum.[Method] Three methods of Cutting Method,Liquid Nitrogen Method and Quartz Sand Method based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage,tender spike and old leaf at heading stage.The obtained DNAs were detected by absorbance detection,agarose gel and PCR amplification.[Result] Cutting Method performed better than the other two methods compared in purity,extracting cycle and cost,accordingly more suitable for PCR amplification.The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua.