Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with <Emphasis Type="Italic">Enterococcus</Emphasis> sp. |
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| Authors: | Maurício L Martins Felipe N Vieira Gabriela T Jerônimo José L P Mouriño Geovana Dotta Gisele M Speck Adolfo J M Bezerra Fabíola S Pedrotti Celso C Buglione-Neto Jr" target="_blank">Gilberto PereiraJr |
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| Institution: | (1) Laboratory of Diagnosis and Pathology in Aquaculture, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. SC 404, Km 3, Florianópolis, SC, 88040-900, Brazil;(2) Aquaculture Department, Federal University of Santa Catarina (CCA, UFSC), Rod. SC 404, Km 3, Florianópolis, SC, 88040-900, Brazil;(3) Marine Shrimp Laboratory, Aquaculture Department, CCA, UFSC, Beco dos Coroas, Barra da Lagoa, Florianópolis, SC, 88062-601, Brazil |
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| Abstract: | This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with
1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for
white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers
of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities
in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml. |
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| Keywords: | Infection Leukocyte Phagocytosis Tilapia |
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