<Emphasis Type="Italic">N</Emphasis>,<Emphasis Type="Italic">N</Emphasis>-dimethylsphingosine,an inhibitor of sphingosine kinase,induces phytoalexin production and hypersensitive cell death of Solanaceae plants without generation of reactive oxygen species |
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Authors: | Sadao Uruma Yusuke Shibata Daigo Takemoto Kazuhito Kawakita |
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Institution: | (1) Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan |
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Abstract: | Plant recognition of elicitors derived from pathogens induces various resistant reactions, including production of reactive
oxygen species, hypersensitive cell death and accumulation of phytoalexins. Previously, we isolated a ceramide elicitor from
Phytophthora infestans, which activates O2
− production of potato suspension-cultured cells. In this study, we employed nine ceramide-related chemicals to test their
elicitor activity. Although, none of the tested chemicals induced O2
− production, N,N-dimethylsphingosine (DMS) induced accumulation of phytoalexin in potato tubers. In potato, tobacco and Nicotiana benthamiana, DMS also induced rapid cell death. DMS-treated potato cells stained with 4′,6-diamidino-2-phenylindole (DAPI) showed chromatin
condensation, and isolated DNA from DMS-treated cells had ladder pattern, confirming that DMS-induced plant cell death is
a hypersensitive reaction-like programmed cell death. Involvement of ceramide signaling in induction of plant defense reactions
is discussed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Hypersensitive cell death Phytoalexin Phytophthora infestans Potato Reactive oxygen species N N-dimethylsphingosine |
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