741杨离体叶片再生及抗虫基因转化

用７４１杨［Ｐｏｐｕｌｕｓａｌｂａ（ｐ．ｄａｖｉｄｉａｎａ×Ｐ．ｓｉｍｏｎｉｉ）×Ｐ．ｔｏｍｅｎｔｏｓａ）］试管植株无菌叶片作外植体，经研究，筛选了诱导不定芽分化、增殖和生根培养基；暗培养５～１５ｄ能提高不定芽分化的诱导率。无根或生根试管植株两类叶片均可用作外植体。建立了７４１杨最佳转化和再生系统。用含有完全改造的Ｂｔ基因表达载体ｐＢ４８．７转化优良杨树新品种－７４１杨，经在含有卡那霉素培养基上选择和ＰＣＲ检测已获得初步确定的转基因植株。

Plant Regeneration of Excised Leaf from 741 Poplar and Transformation with Insect-Resistant B.t.toxin Gene

Using leaves from aseptuly plantets of 741 poplar Populus alba×(P.davidiana×P.simonii)× P.tomentosa] as the explants, the optimiu conditions for the induction of adventitious bud differentiation, multiplication and rooting were selected in this study. Dark culture for 5- 15 days could increase the rate of adventitious bus differentiation. meanwhile,the status of adventitious bud in rooted and rootless plantlets in vitro were compared. Finally, a best system for iran formation and regeneration of 741. poplar plants was established. The modified B. t. eaxin gene carried on PB 48. 7 was used to transform elite variety poplar 741.According to the selection in kanamycin contained media and PCR test,the plantlets with the transformed gene were created.