降解菌N80对土壤中氯嘧磺隆降解效果的研究

以N80为供试菌株,研究了N80对草甸黑土中氯嘧磺隆的降解能力,并测定了N80菌株的接种量、底物氯嘧磺隆初始量、土壤中的水分及土壤不同类型对氯嘧磺隆的降解效果。结果表明:当底物的浓度为20 mg/kg时,在灭菌草甸黑土中添加N80菌株5×1014CFU/g,N80对底物氯嘧磺隆的降解率由32.5%增加到87.7%,而在没有灭菌的土壤中添加N80菌株后,底物氯嘧磺隆的降解率由47.5%提高到94.4%。同时对不同性质的棕壤土和草甸黑土接种降解菌N80,第30天测定棕壤土和草甸黑土中的氯嘧磺隆降解率分别为49%、87.5%。底物氯嘧磺隆含量20 mg/kg、N80接种量为5×1014CFU/g、土壤水分含量30%时,在草甸黑土中N80对氯嘧磺隆除草剂的降解效果较好。

Degradation Effect of Degrading Bacterium N80 on Chlorimuron-ethyl in Soil

Objective]Research the degradation effect of chlorimuron-ethyl in soil by degrading bacteria N80.Method]In this study, the chlorimuron-ethyl degradation bacteria N80 uesd as a test strain, has been carried out degrading chlorimuron-ethyl with the meadow black soil inoculation under greenhouse condition in order to analysis the degradation activity of the strain. And the degradation effect of chlorimuron-ethyl was measured with different initial dosege, inoculum concentration, soil moisture and property. Result]The results indicate that chlorimuron-ethyl degradation rate increased from 32.5% to 87.7% when the chlorimuron-ethyl content was 2mg/kg, and with the strain N80 5×1014 CFU/g in the sterile soil. In contrast, the chlorimuron-ethyl degradation rate increased from 47.5% to 94.4% in the non-sterile soil. Meanwhile, the chlorimuron-ethyl degradation rate is 49% in brown soil inoculated with the strain N80 , and 87.5% in meadow black soil with strain N80 respectively. The findings suggest the chlorimuron-ethyl is degraded much better while the chlorimuron-ethyl content was 20 mg/kg,with strain N80 inoculum 5×1014 CFU/g, moisture content 30%,and the chlorimuron-ethyl degradation effect is much higher in meadow black soil than in brown soil further. Conclusion]Degrading bacteria N80 can markedly improve the degradation speed of chlorimuron-ethyl in soils.