鸭副粘病毒人工感染鸭的病理组织学研究

为研究鸭副粘病毒致病机理及病理组织变化。本研究将20日龄非免疫健康建湖麻鸭60羽,随机均分为试验组和对照组。试验组鸭皮下接种1:5稀释的鸭副粘病毒WF00D株SPF鸡胚绒尿液0.5mL/羽,对照组鸭皮下注射等量灭菌生理盐水,并分别置于25℃隔离环境下饲养与观察。于接种后第4d、8d、12d、16d和第20d,两组均随机取4羽鸭进行剖检,观察各器官的大体病变,同时,观察病理组织学变化。结果显示:试验组鸭除个体发育呈明显差异外,内脏器官在观察期间均有较轻微的炎性水肿或出血;病理组织学变化主要为器官充血或出血、炎性细胞浸润、细胞颗粒变性;超微病理学变化主要为细胞器变性,胞浆局灶性坏死、线粒体空泡化、粗面内质网脱颗粒。研究表明其病理演变过程具有一定的规律性。本研究为鸭副粘病毒流行规律的探究及致病机理的深入研究提供了依据。

Pathohistological studies on ducks infected with NDV of duck origin

To investigate pathohistological changes in ducks infected by NDV of duck origin, healthy Jianhu ducklings were inoculated subcutaneously with 0.5 mL SPF chicken embryos chorioallantoic fluid infected by NDV(WFOOD strain). These ducklings were raised and observed in housing with 25 ℃ slaughtered randomly at the 4th, 8th, 12th, 16th and 20th day of post-injection, and the pathological changes were observed by light microscope and transmission electron microscope (TEM) after autopsy. The clinical symptom of ducks was minor despite significant differences in body size and weight. Relatively mild inflammatory edema or hem-orrhage was seen in the internal organs. Pathohistological changes showed congestive organ or bleeding, inflammatory cell infiltra-tion, cell particles degeneration. Ultrastructural changes included cell degeneration, focal endochylema necrosis, vacuolization of mitochondria, rough endoplasmic reticulum degranulation. The pathological changes progressed gradually during first 16 days However lesions in thymus, bursa Fabricii, spleen and digestive tube on the 16th and 20th day were reduced. The pathohistological changes then improved gradually in most of the major organs except of the liver, kidney and lung lesions.