| 1种适用于转基因玉米PCR检测的DNA快速提取方法 |
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| 引用本文： | 戴 军 汪 海 朱 莉等. 1种适用于转基因玉米PCR检测的DNA快速提取方法[J]. 安徽农业科学, 2014, 0(12): 3494-3496 |
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| 作者姓名： | 戴 军 汪 海 朱 莉等 |
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| 作者单位： | [1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]中国农业科学院生物技术研究所,北京100081 |
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| 基金项目： | 转基因生物新品种培育重大专项（批准号：2013ZX08003-001）;国家自然科学基金（批准号：30970231）. |
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| 摘 要： | [目的]建立一种快速提取玉米基因组DNA的方法。[方法]对改良CTAB法进行进一步简化,不需要进行沉淀、洗涤、溶解和RNA消化等步骤,建立一种快速提取玉米基因组DNA的方法。[结果]该方法获得的DNA完整性及PCR扩增效果与试剂盒提取方法相比无明显差异;经过对样品ELISA检验可知,该方法得到的DNA用于PCR检测结果准确。[结论]该方法能够满足转基因玉米PCR检测中快速、高效、准确、高通量的要求。
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| 关 键 词： | 转基因玉米 基因组DNA 快速提取 PCR检测 |
A Rapid DNA Extraction Method Applying to PCR Detection of Transgenic Maize |
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| DAI Jun,LANG Zhi-hong. A Rapid DNA Extraction Method Applying to PCR Detection of Transgenic Maize[J]. Journal of Anhui Agricultural Sciences, 2014, 0(12): 3494-3496 |
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| Authors: | DAI Jun LANG Zhi-hong |
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| Affiliation: | et al (School of Life Science and Engineering, Southwest Science and Technology University, Mianyang, Si- chuan 621010; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081 ) |
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| Abstract: | [ Objective ] To establish a fast method to extract maize genome DNA. [ Method ] Improved CTAB method was further simplified, a method for rapid extraction of the maize genome DNA was established without the steps of alcohol precipitation, washing, dissolving and RNA digestion. [ Result] The DNA integrity and the PCR amplification using this method showed no significant difference from the plant ge- nomic DNA extraction kit method. Furthermore, it was same detection results with ELISA analysis and with PCR detection using DNA template obtained by this method. [ Conclusion] Therefore, this method is a rapid, efficient, accurate, high-throughput method that meets the PCR de- tection requirements of genetically modified maize. |
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| Keywords: | uTransgenic maize Genomic DNA Rapid extraction PCR detection |
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