| 一个金黄色葡萄球菌蛋白磷酸酯酶基因的克隆表达及酶活性测定 |
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| 引用本文： | 吕茂利,于立权,秦学功,崔玉东. 一个金黄色葡萄球菌蛋白磷酸酯酶基因的克隆表达及酶活性测定[J]. 黑龙江八一农垦大学学报, 2016, 0(4): 72-74,104. DOI: 10.3969/j.issn.1002-2090.2016.04.016 |
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| 作者姓名： | 吕茂利 于立权 秦学功 崔玉东 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 基金项目： | 黑龙江八一农垦大学“博士科研启动基金”(校启2011YB-15);国家自然基金面上项目(31072120) |
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| 摘 要： | 金黄色葡萄球菌是一种引起人畜多种感染的主要病原菌,开发治疗金黄色葡萄球菌感染的新药途径之一是药物寻靶。研究克隆了一个金黄色葡萄球菌基因,并进行了蛋白诱导表达和酶活性测定。结果表明,该基因编码的蛋白具有丝氨酸/苏氨酸蛋白磷酸酯酶活性。实验结果为进一步挖掘该蛋白作为药物靶标的潜在功能奠定了基础。
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| 关 键 词： | 金黄色葡萄球菌 蛋白磷酸酯酶 活性测定 |
Cloning,Expression and Enzyme Assay of Staphylococcus aureus Protein Phosphatase Gene |
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| Lv Maoli;Yu Liquan;Qin Xuegong;Cui Yudong. Cloning,Expression and Enzyme Assay of Staphylococcus aureus Protein Phosphatase Gene[J]. journal of heilongjiang bayi agricultural university, 2016, 0(4): 72-74,104. DOI: 10.3969/j.issn.1002-2090.2016.04.016 |
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| Authors: | Lv Maoli Yu Liquan Qin Xuegong Cui Yudong |
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| Affiliation: | Lv Maoli;Yu Liquan;Qin Xuegong;Cui Yudong;College of Life Science and Technology,Heilongjiang Bayi Agricultural University; |
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| Abstract: | Staphylococcus aureus was an important pathogen causing various infections of human and livestock. Targeting of a drug was a new approach of developing new drugs against S. aureus. A S. aureus gene was cloned,then induced protein was expressed and enzyme assay was conducted. The results showed that the recombinant protein coded by the gene had serine/threonine protein phosphatase activity. This work provided a reference points for further investigating the protein as target for a drug. |
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| Keywords: | Staphylococcus aureus protein phosphatase enzyme assay |
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