复合抗菌肽对山羊瘤胃发酵和酶活性的影响

本试验旨在探讨复合抗菌肽对饲喂不同精料饲粮山羊瘤胃发酵和酶活性的影响。选取18只4月龄雄性山羊,随机分3组,每组6只。对照组(Ⅰ组)、高精料组(Ⅱ组)、高精料抗菌肽组(Ⅲ组)分别饲喂300、600和600 g/(只·d)精料,同时Ⅲ组在精料中添加3.0 g/(只·d)复合抗菌肽。试验期为60 d。结果表明:1)与Ⅰ组相比,Ⅱ组瘤胃液乙酸、总挥发性脂肪酸(T-VFA)、甲烷(CH4)、氨态氮(NH3-N)、尿素氮、微生物蛋白(MCP)浓度与(乙酸+丁酸)/丙酸及木聚糖酶、脂肪酶活性极显著或显著增加(P0.01或P0.05),羧甲基纤维素酶(CMCase)、β-葡萄糖苷酶和淀粉酶活性极显著或显著降低(P0.01或P0.05),丙酸、丁酸浓度及果胶酶、中性蛋白酶活性无显著变化(P0.05)。2)与Ⅱ组相比,Ⅲ组瘤胃液丙酸、丁酸、NH3-N浓度及CMCase、果胶酶、中性蛋白酶及脂肪酶活性无显著变化(P0.05),乙酸、T-VFA、CH4、尿素氮浓度与(乙酸+丁酸)/丙酸及木聚糖酶活性极显著或显著降低(P0.01或P0.05),MCP浓度及β-葡萄糖苷酶、淀粉酶活性极显著或显著增加(P0.01或P0.05)。由此说明,复合抗菌肽可调节山羊瘤胃发酵模式,提高饲料利用率,是理想的饲料添加剂。

Effects of Compound Antimicrobial Peptides on Ruminal Fermentation and Enzyme Activities of Goats

This experiment was evaluated to study the effects of compound antimicrobial peptides (C-AMPs) on ruminal fermentation and enzyme activities of goats fed diets with different concentrates.Eighteen 4-month-old male goats were randomly divided into 3 groups and each group had 6 goats.The groups included control group (group Ⅰ), high-concentrate group (group Ⅱ), and high-concentrate antimicrobial peptide group (group Ⅲ), and fed with 300, 600 and 600 g concentrate per head per day, respectively.Moreover, the concentrate of group Ⅲ supplemented with 3.0 g C-AMPs per head per day.The experiment lasted for 60 days.The results showed as follows: relative to the group Ⅰ, the group Ⅱ showed a significant increase in the concentrations of acetate, total volatile fatty acids (T-VFA), methane (CH4), ammoniacal nitrogen (NH3-N), urea nitrogen, microprotein (MCP), the ratio of (acetate+butyrate) to propionate, the activities of xylanase and lipase in rumen fluid (P<0.01 or P<0.05), and showed a significant decrease in the activities of carboxymethyl cellulose (CMCase), β-glucosidase and amylase (P<0.01 or P<0.05), but did not show a significant change in the concentrations of propionate and butyrate, and the activities of pectinase and neutral protease (P>0.05).Relative to the group Ⅱ, the group Ⅲ had no significant change in the concentrations of propionate, butyrate, NH3-N and the activities of CMCase, pectinase, neutral protease and lipase (P>0.05), but the group Ⅲ showed a significant decrease in the concentrations of acetate, T-VFA, CH4, urea nitrogen, the ratio of (acetate+butyrate) to propionate and the activity of xylanase (P<0.01 or P<0.05), and showed a significant increase in the concentration of MCP and the activities of β-glucosidase and amylase (P<0.01 or P<0.05).The results indicate that dietary C-AMPs can modulate ruminal fermentation model and enhance feed conversion rate, and it can be applied as an ideal feed additive.