丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响

本试验旨在研究饲粮中添加丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响。试验采用单因子设计,选择28日龄、体重相近、健康状况良好的"杜×长×大"断奶仔猪12头,按完全随机区组设计分为2个组,每组6个重复,单栏饲养。对照组饲喂玉米-豆粕型基础饲粮,试验组饲喂基础饲粮+5×10~5CFU/g丁酸梭菌。试验预试期3 d,正试期14 d。结果表明:与对照组相比,试验组仔猪平均日增重显著提高7.83%(P0.05)、料重比降低5.26%(P0.05);试验组仔猪空肠NOD样受体蛋白(NLRP)3(P0.05)、NLRP6(P0.05)、NLRP12(P0.01)、封闭蛋白1(claudin-1)(P0.01)和紧密连接蛋白2(ZO-2)(P0.05)的mRNA相对表达水平显著或极显著上调,回肠claudin-1和ZO-2的mRNA相对表达水平极显著上调(P0.01);试验组仔猪空肠和回肠中乳酸杆菌数量显著提高(P0.05),空肠中大肠杆菌数量降低2.49%(P0.05),回肠中大肠杆菌数量显著降低(P0.05);试验组仔猪血清白细胞介素(IL)-1β含量降低5.47%、IL-10含量提高25.43%(P0.05)。综上所述,饲粮中添加丁酸梭菌能提高仔猪小肠屏障功能,调节机体免疫和肠道菌群平衡,促进仔猪生长。

Effects of Clostridium butyricum on Growth Performance,Intestinal Barrier Function and Serum Cytokine Contents of Weaned Piglets

This study was aimed to investigate the effects of Clostridium butyricum on growth performance, in-testinal barrier function and serum cytokine contents of weaned piglets. A single factor design was used in this experiment, and 12 Duroc×Landrace×Yorkshire weaned piglets with similar body weight and good health were selected and divided into 2 groups at 28 days of age. Each group was divided into 6 replicates and each pig was fed in single column. Pigs in control group were fed a corn-soybean type basal diet, and those in experimental group were fed the basal diet supplemented with 5×105 CFU/g Clostridium butyricum for 14 days after 3 days adaption. The results showed as follows: compared with the control group, the average daily gain of piglets in experimental group was significantly increased by 7.83% (P<0.05), and the ratio of feed to gain was de-creased by 5.26% (P>0.05); the mRNA relative expression levels of NOD like receptor protein (NLRP) 3 (P<0.05), NLRP6 (P<0.05), NLRP12 (P<0.01), claudin-1 (P<0.01) and zonula occludens protein 2 ( ZO-2) ( P<0.05) were significantly or extremely significantly increased in jejunum and the mRNA relative expression levels of claudin-1 and ZO-2 were extremely significantly increased ( P<0.01) in ileum of piglets in experimental group; the number of Lactobacilli in the jejunum and ileum of piglets in experimental group was significantly increased ( P<0.05) , and the number of Escherichia coli in the jejunum of piglets in experimental group was decreased by 2.49% ( P>0.05) , while the number of Escherichia coli in the ileum of piglets in ex-perimental group was significantly decreased (P<0.05); the serum interleukin (IL)-1β content of piglets in experimental group was decreased by 5.47% ( P>0.05) , but the serum IL-10 content of piglets in experimental group was increased by 25.43% (P>0.05). In conclusion, dietary Clostridium butyricum can improve small intestinal barrier function of weaned piglets, regulate body immunity and the balance of intestinal flora, and thus promote weaned piglets growth.