应用Illumina-MiSeq高通量测序技术分析脱氧雪腐镰刀菌烯醇对小鼠肠道菌群的影响

本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)对小鼠肠道微生物多样性、物种丰度的影响。选用平均体重为20 g的BALB/c小鼠40只,随机分成对照组(灌胃灭菌生理盐水)和DON组(灌胃1.8 mg/kg BW DON),每组20个重复,每个重复1只小鼠,连续灌服28 d。试验结束后,收集新鲜粪便,每组随机选取3份,用Illumina-Mi Seq高通量测序技术分析微生物群落结构和组成的变化。结果表明,1)与对照组相比,DON组测序数量减少(P0.05)。2)DON组降低了Alpha、Beta多样性,Shannon指数较对照组显著降低(P0.05)。3)在门水平,与对照组相比,DON组显著降低了拟杆菌门(Bacteroidetes)和脱铁杆菌门(Deferribacteres)的物种丰度(P0.05),显著提高了变形菌门(Proteobacteria)的物种丰度(P0.05)。在属水平,与对照组相比,DON组显著降低了副类杆菌属(Parabacteroides)、理研菌属(Rikenella)、Algoriphagus、Mucispirillum、嗜甲基菌属(Methylophilus)、Francisella的物种丰度(P0.05),显著提高了梭菌属(Clostridium)、Robinsoniella、A llobaculum、A kkermansia的物种丰度(P0.05)。4)聚类分析显示,DON组与对照组的肠道微生物相似性降低。由此可见,DON能显著影响小鼠肠道微生物菌群多样性及物种丰度,提示DON致肠道损伤与这些肠道菌群的变化密切相关。

Effects of Deoxynivalenol on Intestinal Microbiota of Mice Analyzed by Illumina-MiSeq High-Throughput Sequencing Technology

This study was conducted to investigate the effects of deoxynivalenol ( DON) on bacterial diversity and species abundance of intestinal microbiota. A total of 40 BALB/c mice weighted about 20 g were randomly allotted to control group ( intragastric gavage sterilized saline) and DON group ( intragastric gavage 1.8 mg/kg BW DON) with 20 replicates per group and 1 mouse per replicate. The mice were fed by intragastric adminis?tration for 28 days. After the test, fresh fecal samples were collected and 3 samples in each group were ran?domly selected for investigating the microbial community and composition by using Illumina?MiSeq high?throughput sequencing technology. The results showed as follows: 1) the quantities of sequences observed in DON group was less than that in control group ( P>0.05) . 2) The Alpha and Beta diversity were respectively reduced in DON group, and the Shannon index was also significantly decreased compared with the control group ( P<0.05) . 3) At the phylum level, compared with the control group, the abundances of Bacteroidetes and Deferribacteres in DON group were significantly decreased ( P<0.05) , while the abundance of Proteobac?teria was significantly increased (P<0.05). At the genus level, compared with the control group, the abun?dances of Parabacteroides, Rikenella, Algoriphagus, Mucispirillum, Methylophilus and Francisella in DON group were significantly decreased ( P<0.05) , but the abundances of Clostridium, Robinsoniella, Allobaculum and Akkermansia in DON group were significantly increased (P<0.05). 4) The cluster analysis showed low similarity of intestinal microflora between the control and DON group. In conclusion, the bacterial diversity and species abundance of intestinal microbiota can be influenced by DON, and it is suggested that the intestinal le?sions caused by consumption of DON may be related with the change of intestinal bacteria communities.