猪圆环病毒II型PCR检测方法的研究

【目的】建立快速、简便、特异的检测猪圆环病毒II型的PCR方法；【方法】根据已发表的猪圆环病毒II型基因序列设计合成了一对引物，用酚-氯仿法抽提可疑病料中的病毒DNA，应用PCR技术对猪圆环病毒进行基因扩增，PCR产物进行序列测定；以猪瘟病毒、猪伪狂犬病毒、猪细小病毒为对照，进行特异性试验；取部分病料进行重复性试验；【结果】PCR方法扩增出了长度为702bp的片段，扩增产物经测序和序列分析表明扩增的序列为PCV2序列；猪瘟病毒、猪伪狂犬病毒、猪细小病毒的PCR扩增均为阴性,与预期结果一致；部分病料PCR重复检测5次，检测结果完全一致；【结论】PCR检测方法可以对猪圆环病毒病作出敏感、特异、准确地诊断，该方法检测的基因最低模板量为2×10-5 ng/ml

Detection of Procine Circovirus Type 2 by PCR Technique

The paper studied the effect of different level of cobalt sulfate on fresh grass yield and nutrient quality of alfalfa by leaf surface spurting.The result indicated that,Spurting cobalt sulfate could remarkably(P < 0.05)increase the fresh grass yield of alfalfa and the leaf stem ratio(LS).The effect of the concentration of 300 mg/kg treatment is best,in compared to CK treatment,the fresh grass yield increased by 25%.Different levels of cobalt sulfate affect the nutrient content differently.The contents of crude protein(CP)and P was increased with the applied Co amount increased,while there was on significantly different on content of crude fiber(CF),nitrogen free extract(NFE),crude ash(CA),and the content of crude fat(CF)was decreased with the applied Co amount increased.When the Co concentration was 300 mg/kg,the yield of crude protein(CP),crude ash(CA),P,NFE was reach max.,among the all of treatment,and significantly higher than that of CK treatment,the yield of crude fiber and Ca of treatment Co2 was reach max.,but there were no significantly different to CK.and crude fat was Co1 treatment.