云南省烟草青枯病危害调查与病原菌分离

为了了解云南省烟草青枯病的危害情况，指导病害预测预报与综合防治；2004年至2006年，对云南省烟草青枯病的发生进行了调查，对田间采集的烟草茎下部黑色坏死症状样品进行了病原菌分离。在文山州文山县、砚山县、广南县、西畴县和麻栗坡县主要栽烟乡镇的重病田块，茎下部黑色坏死病株86.3%感染青枯病，27.7%感染黑胫病，其中包括14%病株复合感染青枯病和黑胫病。从云南省12个县(市)采集的258个烟草病样中分离青枯雷尔氏菌株219个，病原菌回接试验和ELISA检测结果表明文山州、保山市的烟草茎下部黑色坏死症状病害，大部分为烟草青枯病，伴随部分黑胫病。

The Disease Survey and pathogen Isolation of Tobacco Bacterial Wilt in Yunnan Province, China

From 2004 to 2006, tobacco bacterial wilt disease survey and pathogen isolation were carried out in Yunnan province. In Wenshan prefecture the marjor tobacco plant county, such as wenshan, Yanshan, Guangnan, Xichou and Malipo county, 86.3% disease plant with lower stem black necrotic affect bacterial wilt, 27.7% affect black shank, among 14% plant mixed effect bacterial wilt and black shank. 219 strains of Ralstonia solanacearum are isolated from 258 samples gathered from 12 counties of Yunnan provinces. The pathogen backward inoculation test and ELISA test show that tobacco lower stem black necrotic in Wenshan and Baoshan prefecture is mostly associated with tobacco bacterial wilt, partial with black shank.