| 中国锦紫苏类病毒的检测及其分子生物学特征研究 |
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| 引用本文： | 苏前富,郭瑞,成卓敏,SANO TERUO,李世访.中国锦紫苏类病毒的检测及其分子生物学特征研究[J].植物病理学报,2006,36(3):226-231. |
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| 作者姓名： | 苏前富 郭瑞 成卓敏 SANO TERUO 李世访 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京 100094;2. 吉林大学植物科学学院, 长春 130062;3. Laboratory of Phytopathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036, Japan |
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| 基金项目： | 高比容电子铝箔的研究开发与应用项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | 从无明显症状表现的11株锦紫苏叶片中抽提低分子量的RNA,经Return-PAGE、RT-PCR和Dot-blot hybridization检测,结果表明11株锦紫苏全部带有锦紫苏类病毒(Coleus blumei viroid,CBVd)。将部分PCR产物克隆到pGEM-3Zf(+)载体上并进行DNA序列测定。序列分析结果,所克隆的序列(GenBank登录号分别为DQ178395、DQ178396、DQ178397、DQ178398和DQ178399)与GenBank中报道的锦紫苏类病毒1号(CBVd-1)序列同源性为85.23%~99.20%。从市场上购买的锦紫苏种子经Return-PAGE和RT-PCR检测不携带锦紫苏类病毒。这是中国发生的锦紫苏类病毒的首次报道。
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| 关 键 词： | 锦紫苏类病毒 正反向电泳 斑点杂交 RT-PCR |
| 文章编号： | 0412-0914(2006)03-0226-06 |
| 修稿时间： | 2005年9月5日 |
Detection and molecular characterization of Coleus blumei viroid in China |
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| SU Qian-fu,GUO Rui,CHENG Zhuo-min,SANO TERUO,LI Shi-fang.Detection and molecular characterization of Coleus blumei viroid in China[J].Acta Phytopathologica Sinica,2006,36(3):226-231. |
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| Authors: | SU Qian-fu GUO Rui CHENG Zhuo-min SANO TERUO LI Shi-fang |
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| Institution: | 1. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;2. College of Plant Sciences, Jilin University, Changchun 130062, China;3. Laboratory of Phytopathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036, Japan |
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| Abstract: | The low molecular weight RNAs from 11 coleus plants were characterized by Return-PAGE,RT-PCR and Dot-blot hybridization.The results showed that all the 11 coleus plants were infected by Coleus blumei viroid.The amplified products of RT-PCR were cloned into vector pGEM-3Zf(+) and sequenced.5 sequences variants were registered in GenBank DQ178395,DQ178396,DQ178397,DQ178398 and DQ178399,and compared with reported sequences of CBVd-1 in GenBank showing 85.23%-99.20% identity.No Coleus blumei viroid 1 detected from coleus seeds by RT-PCR and Return-PAGE.This is the first report on the occurrence of CBVd in China. |
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| Keywords: | RT-PCR |
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