动物源沙门菌耐药性调查及Ⅰ类整合子的检测

采用肉汤微量稀释法,选用22种常用抗菌药物,对98株动物源沙门菌进行药敏试验,结果显示:菌株对磺胺类、四环素类药物及萘啶酸普遍耐药,对氨基糖苷类药物耐药率较低,对氟喹诺酮类药物高度敏感;菌株多重耐药率为67.35%(66/98).采用PCR方法检测菌株Ⅰ类整合子的流行情况,并分析其携带的耐药基因盒.结果98株沙门菌中Ⅰ类整合子的检出率为50.0%(49/98),并且均携带耐药基因盒,基因盒以dfrA17-aadA5的组合形式最为常见;Ⅰ类整合子阳性菌株的多重耐药率为95.92%(47/49),阴性菌株的多重耐药率为38.78%(19/49).上述结果表明Ⅰ类整合子普遍存在于兽医临床耐药沙门菌中,其流行与菌株多重耐药性具有一定的相关性.

Detection of antimicrobial resistance and class 1 integrons among Salmonella isolates from animals

The MICSof 22 kinds of autimicrobial agents to Salmonella isolates from animals were determined by the broth microdiluaon.The results showed that these isolates were high resistant to sulfanilamide,tetracyclines and Nalidixic acid,whereas low resistant to aminoglycosides,and almost all of isolotes were sensitive to fluoroquinolones;66 isolates(67.35%) were multidrug resistant.PCR and DNA sequencing were used for characterization of class 1 integrons and gene cassettes.50.0%(49/98) of the isolates were posit...