| 非豆科作物共生固氮研究Ⅰ、菠菜根瘤内生菌的分离与回接 |
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| 引用本文： | 杨振华,张谷曼.非豆科作物共生固氮研究Ⅰ、菠菜根瘤内生菌的分离与回接[J].福建农林大学学报(自然科学版),1985(3). |
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| 作者姓名： | 杨振华 张谷曼 |
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| 作者单位： | 福建农学院园艺系，福建农学院园艺系 |
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| 摘 要： | 从菠菜(Spinacia oleracea)根瘤上分离到一种细菌内生菌84G,获得纯培养。该菌系好气性、快生型,能固定大气氮。回接试验表明,该菌能感染菠菜幼根,接种后15～25天结根瘤,根瘤的固氮酶活力达4.01μmol C_2H_4/g·鲜重·h。
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STUDIES ON SYMBIOTIC NITROGEN FIXATION OF NON-LEGUMINOUS CROPS Ⅰ.ISOLATION AND INOCULATION OF NODULE ENDOPHYTE FROM SPINACIA OLERACEA |
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| Abstract: | A bacterium endophyte 84G from root nodules of Spinacia oleracea has been isolated as pure culture.The strain is aerobic,fast-growing.Moreover the strain is capable of fixing molecular nitrogen.Back inoculation experiments demonstrated its ability of infecting young roots of spinach.The root nodules appeared in 15-25 days after inoculation.The root nodules possessed the capability of N_2-fixation with the activity reaching to 4.01 μmol ethylene/ g.fresh weight·h. |
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