Unique viral capsid assembly protein gene (g20) of cyanophages in the floodwater of a Japanese paddy field |
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Authors: | Guanghua Wang Jun Murase Susumu Asakawa Makoto Kimura |
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Institution: | (1) Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan;(2) Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China |
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Abstract: | In order to evaluate the genetic diversity of cyanophage communities of rice fields, viral capsid assembly protein gene (g20) was amplified with primers CPS1 and CPS8. The DNA was extracted three times from viral concentrates obtained from floodwater
samples collected in each of four different plots (no fertilizer; P and K chemical fertilizers; N, P, and K chemical fertilizers;
and chemical fertilizers with compost). Denaturing gradient gel electrophoresis (DGGE) gave different g20 clones. The sequencing of DGGE bands revealed that the g20 genes of the floodwater were divergent and that the majority of clones formed several unique groups. However, they were more
closely related to g20 sequences from freshwaters than to those from marine waters, suggesting that g20 genes in terrestrial aquatic environments are different from those in marine environments. |
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