| 犊牛源乳酸菌的筛选及抑菌性能研究 |
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| 引用本文： | 郭奇苑,孙文,徐基利,许丽.犊牛源乳酸菌的筛选及抑菌性能研究[J].中国饲料,2010(6). |
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| 作者姓名： | 郭奇苑 孙文 徐基利 许丽 |
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| 作者单位： | 东北农业大学动物科学技术学院 |
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| 基金项目： | 国家科技支撑计划"东北农区奶业集约化生产技术集成及产业示范"，黑龙江省科技攻关计划"功能性饲料及粗饲料高效利用关键技术研究" |
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| 摘 要： | 从15日龄健康犊牛的肠道黏膜和食糜分离得到31株革兰氏阳性、无芽孢乳酸菌。通过耐消化道环境筛选得到4株乳酸菌M5、M7、SB1和Z4,在pH=2条件下处理2h后的存活率分别为39.24%、28.08%、44.20%、23.28%,有较强的耐牛胆盐能力,经人工胃液处理后各菌的存活率均可达到30%以上,且活菌数超过108CFU/mL。能抵御体外模拟的胃肠道逆环境的影响,且对大肠杆菌的抑制作用明显,抑菌圈直径为16～21mm。
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| 关 键 词： | 犊牛 乳酸菌 耐受性 抑菌作用 |
Screening of lactic acid bacteria isolated from calf and study on its bacteriostasis |
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| Guo Qiyuan,Sun Wen,Xu Jili,et al..Screening of lactic acid bacteria isolated from calf and study on its bacteriostasis[J].China Feed,2010(6). |
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| Authors: | Guo Qiyuan Sun Wen Xu Jili |
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| Abstract: | Thirty one lactic acid bacteria strains,Gram staining masccline and non-spore,were isolated form different intestinal tract mucosa and chyme of 15 days healthy calves.Four strains,M5,M7,SB1 and Z4 were selected through the test of tolerance acid,tolerance bile salt with different concentration and artificial gastric juice.The survivabilities of strain M5,M7,SB1 and Z4 were 39.24 %,28.08 %,44.20 % and 23.28 % respectively when treated with acid of pH=2 for 2 hours.All of the 4 strains had commendable intoler... |
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| Keywords: | calf lactic acid bacteria tolerance ability bacteriostasis |
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