| 鸭源H5亚型禽流感全禽源分子标记疫苗候选株的构建 |
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| 引用本文： | 张文俊,薛涛,吴小伟,唐应华,彭大新,刘秀梵.鸭源H5亚型禽流感全禽源分子标记疫苗候选株的构建[J].畜牧兽医学报,2011,42(5). |
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| 作者姓名： | 张文俊 薛涛 吴小伟 唐应华 彭大新 刘秀梵 |
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| 作者单位： | 1. 扬州大学农业部畜禽传染病学重点开放实验室,扬州,225009
2. 江苏省农业科学院国家兽用生物制品工程技术研究中心,南京,210014 |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目，国家重点基础研究发展计划(973计划)项目 |
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| 摘 要： | 为了开发用于南方水禽且适合血清学监测的H5亚型禽流感疫苗,作者通过反向遗传技术,删除A/mallard/Huadong/S/2005(H5N1,S株)病毒HA编码多碱性氨基酸序列,使之成为低致病特征,分别与A/duck/England/1/1956(H11N6,E株)和A/Chicken/Shanghi/F/98(H9N2,F株)的NA组合,再以本室禽源高产病毒F株的6个内部片段为骨架,构建全部基因都来自禽流感的疫苗株.成功拯救出2株重组病毒,分别命名为rH5N6/F和rH5N2/F,并引入分子标记N6和N2.重组病毒在鸡胚和MDCK细胞上均具有较好的繁殖能力,且rH5N6/F更适合在鸡胚中生产,对SPF鸡和鸡胚无致病性.重组病毒在clade2.3.4毒株中具有很好的抗原代表性,引入的分子标记有利于血清学监测的区分,为防控水禽H5亚型禽流感提供了良好的疫苗候选株.
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| 关 键 词： | 禽流感病毒 反向遗传 H5 DIVA疫苗 |
Generation of Duck Origin H5 Subtype Avian Influenza Vaccine Candidate with Heterogenous NA Gene |
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| ZHANG Wen-jun,XUE Tao,WU Xiao-wei,TANG Ying-hua,PENG Da-xin,LIU Xiu-fan.Generation of Duck Origin H5 Subtype Avian Influenza Vaccine Candidate with Heterogenous NA Gene[J].Acta Veterinaria et Zootechnica Sinica,2011,42(5). |
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| Authors: | ZHANG Wen-jun XUE Tao WU Xiao-wei TANG Ying-hua PENG Da-xin LIU Xiu-fan |
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| Institution: | ZHANG Wen-jun1,XUE Tao1,WU Xiao-wei1,TANG Ying-hua2,PENG Da-xin1,LIU Xiu-fan1 (1.Key Laboratory of Animal Infectious Diseases,Yangzhou University,Yangzhou 225009,China,2.National Veterinary Biological Medicine Engineering Research Center,Nanjing 210014,China) |
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| Abstract: | The aim of this study was to develop H5 subtype avian influenza vaccine with all genes from avian origin influenza viruses,and provide a DIVA(Differentiating Infected from Vaccinated Animals) strategy for the control of avian influenza.Sequences encode several basic amino acids of HA gene from A/mallard/Huadong/S/2005(H5N1,S strain) were deleted.Using reverse genetics system,we rescued two reassortant viruses(rH5N6/F and rH5N2/F) that contained NA genes from A/duck/England/1/1956(H11N6,E strain) or A/Chicke... |
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| Keywords: | avian influenza virus reverse genetics H5 DIVA vaccine |
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