| 寿星桃种质资源的RAPD分析 |
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| 引用本文： | 程中平,陈志伟,胡春根,邓秀新. 寿星桃种质资源的RAPD分析[J]. 北京林业大学学报, 2002, 24(3): 74-77. DOI: 10.3321/j.issn:1000-1522.2002.03.016 |
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| 作者姓名： | 程中平 陈志伟 胡春根 邓秀新 |
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| 作者单位： | 中国科学院武汉植物研究所;武汉市林业果树研究所;华中农业大学国家作物改良重点实验室 |
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| 基金项目： | 湖北省武汉市青年科技晨光计划;985003074; |
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| 摘 要： | 该研究利用RAPD技术,从分子生物学角度对寿星桃种质进行分析.采用从200个10碱基随机引物筛选的22个引物对10个寿星桃类型的基因组DNA进行扩增,通过对180个扩增位点的谱带的聚类,分析供试寿星桃的系统发育;运用特殊谱带,建立了寿星桃的分子检索表;并对扩增的特殊位点进行统计,提出了重点保存的寿星桃种质.
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| 关 键 词： | 寿星桃 RAPD 系统发育 分子检索表 重点种质保存 |
Analysis of Shouxingtao germplasm revealed by RAPD |
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| Cheng Zhongping, Chen Zhiwei,Hu Chungen,Deng Xiuxin.Wuhan Institute of Botany,Chinese Academy of Science,Wuhan ,P.R.China.. Analysis of Shouxingtao germplasm revealed by RAPD[J]. Journal of Beijing Forestry University, 2002, 24(3): 74-77. DOI: 10.3321/j.issn:1000-1522.2002.03.016 |
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| Authors: | Cheng Zhongping Chen Zhiwei Hu Chungen Deng Xiuxin.Wuhan Institute of Botany Chinese Academy of Science Wuhan P.R.China. |
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| Affiliation: | Cheng Zhongping, Chen Zhiwei,Hu Chungen,Deng Xiuxin.Wuhan Institute of Botany,Chinese Academy of Science,Wuhan 430074,P.R.China. |
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| Abstract: | To analyze Shouxingtaos from the angle of molecular biology, genomic DNAs of 10 types of Shouxingtao (%Amgdalus persica% var.%densa%) were amplified by RAPD with 22 primers selected from 200 arbitrary decamer primers. Phylogenetic relationship was analyzed through cluster of bands in 180 amplified loci. Molecular checking index and conservation of key germplasms were put forward according to special bands. |
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| Keywords: | Shouxingtao (%Amgdalus persica% var. %densa%) RAPD phylogenetic relationship molecular checking index conservation of key germplasm |
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