一种适用于动物与植物总DNA提取的方法——改良CTAB法

关于动物和植物基因组DNA的提取方法国内外有很多报道，但同一方法通常只能较好的从动物或植物提取总DNA。在前人报道的基础上，该研究对常用的CTAB法进行了适当修改，获得了一种从动物和植物中均能有效提取DNA的方法。

A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method

Objective] The study aimed to introduea a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. Method] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the spe-cifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. Result] The clear and orderly bands were observed in gel detection, and the val-ues of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77 - I. 83. The DNAs performed well in PCR amplification. Conclu-sion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.