一步双重PCR法检测梨火疫病原细菌（Erwinia amylovora）

由格兰氏阴性菌 Erwinia amylovora引起的梨火疫病对梨树、苹果树以及一些观赏性植物具有极大的破坏性。研究表明自然界中存在不含有pEA29质粒的E. amylovora菌株，基于质粒pEA29序列设计的分子检测方法则不能检测出该菌株。本研究采用两对引物，分别来自染色体和pEA29质粒，同时在一个反应体系中进行PCR反应，扩增片段长度分别为1.6 kb和1.0 kb，可特异性检测不含有pEA29质粒的菌株。该方法可以简单、快速、准确地检测梨火疫病原细菌。

Development of a duplex PCR for identification of the fire blight pathogen, Erwinia amylovora

Fire blight, cause by the Gram-negative bacterium Erwinia amylovora, is a destructive bacterial disease of pear and apple and other fruit trees and ornamental plants. Molecular detection protocols based on the information of the plasmid pEA29 sequence are impractical when the E. amylovora strains without the plasmid pEA29 exist in nature. This report developed the duplex-PCR method, which combined the primers from chromosomal regions and from the plasmid pEA29 in a PCR reaction, is fast, simple and accurate to identify this plant pathogen.