| 48份新疆野生狗牙根遗传多样性的SRAP分析 |
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| 引用本文： | 李江华,高文伟,阿不来提,张延辉.48份新疆野生狗牙根遗传多样性的SRAP分析[J].新疆农业大学学报,2011,34(4):285-291. |
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| 作者姓名： | 李江华 高文伟 阿不来提 张延辉 |
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| 作者单位： | 1. 新疆草地资源与生态重点实验室新疆农业大学草业与环境科学学院,乌鲁木齐,830052
2. 新疆农业大学农学院,乌鲁木齐,830052 |
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| 基金项目： | 国家自然科学基金项目(31160483); 新疆维吾尔自治区高技术研究发展计划项目(201111122); 教育部博士点基金项目(200807580004); 新疆维吾尔自治区教育厅项目(XJEDU2009S36) |
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| 摘 要： | 采用SRAP标记对48份新疆野生狗牙根材料进行遗传多样性分析,14对SRAP引物共扩增产生了133条带,其中123条显示多态性,因此SRAP标记可以有效地用于狗牙根野生资源遗传多样性评价。在物种水平上,多态位点百分率为77.83%,48份狗牙根材料的GS值为0.276～0.930,平均为0.700,各材料间差异明显,具有较为丰富的遗传多样性;居群间遗传分化系数为0.391 4,表明有39.14%的变异存在于居群间,60.86%的变异存在于居群内,居群内的遗传分化大于居群间的遗传分化;聚类分析表明,48份供试材料相似系数为0.58时可被聚为4类,聚类结果与地理来源有一定的相关性,但也有材料例外。
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| 关 键 词： | 狗牙根 遗传多样性 SRAP |
SRAP Analysis on Genetic Diversity of 48 Xinjiang Wild Bermudagrass |
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| LI Jiang-hua,GAO Wen-wei,Abulaiti,ZHANG Yan-hui.SRAP Analysis on Genetic Diversity of 48 Xinjiang Wild Bermudagrass[J].Journal of Xinjiang Agricultural University,2011,34(4):285-291. |
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| Authors: | LI Jiang-hua GAO Wen-wei Abulaiti ZHANG Yan-hui |
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| Institution: | LI Jiang-hua1,GAO Wen-wei2,Abulaiti1,ZHANG Yan-hui1 (1.Key Laboratory of Grassland Resource and Ecology of Xinjiang,College of Pratacultural and Environmental Sciences,Xinjiang Agricultural University,Urumqi 830052,China,2.College of Agronomy,China) |
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| Abstract: | The genetic diversity of Cynodon dactylon L.Pers from Xinjiang was investigated by using SRAP marker,133 bands were amplified by 14 pairs of informative and reliable primers,of which 123 were polymorphic.So,SRAP marker can be used to estimate genetic diversity of Cynodon dactylon L.effectively.At species level,percentage of polymorphic sites is 77.83%;GS value of 48 Cynodon dactylon materials were 0.276-0.930,whose average was 0.700.There were significant differences among all the materiats,with richer gene... |
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| Keywords: | Cynodon dactylon wild resources genetic diversity SRAP |
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