| 紫外线诱变选育苜蓿叶象甲高毒力白僵菌 |
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| 引用本文： | 韩慧,吴小虎,李益,李克梅,赵莉.紫外线诱变选育苜蓿叶象甲高毒力白僵菌[J].新疆农业科学,2011,48(7):1273-1276. |
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| 作者姓名： | 韩慧 吴小虎 李益 李克梅 赵莉 |
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| 作者单位： | 新疆农业大学农学院,新疆草地资源与生态实验室,乌鲁木齐830052 |
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| 基金项目： | 新疆农业大学大学生自主创新项目; 牧草产业技术体系建设专项资金(CARS-35); 国家特色专业建设点大学生创作项目(tszyp1201009) |
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| 摘 要： | 目的]选育出对苜蓿叶象甲高毒力的白僵菌.方法]利用紫外线对前期筛选出的白僵菌菌株照射10和30min进行诱变,选出高几丁质酶突变株,再用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫进行毒力测定.结果]用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫室内毒力测定:处理9d后,诱导菌株Bb- 10- 12校正死亡率为100％,Bb -...
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| 关 键 词： | 苜蓿叶象甲 紫外诱变 白僵菌 毒力测定 |
Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis |
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| HAN Hui,WU Xiao-hu,LI Yi,LI Ke-mei,ZHAO Li.Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis[J].Xinjiang Agricultural Sciences,2011,48(7):1273-1276. |
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| Authors: | HAN Hui WU Xiao-hu LI Yi LI Ke-mei ZHAO Li |
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| Institution: | HAN Hui,WU Xiao-hu,LI Yi,LI Ke-mei,ZHAO Li(Agronomy College of Xinjiang Agriculture University/Xinjiang Laboratory of Grassland Resources and Ecology,Urumqi 830052,China) |
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| Abstract: | 【Objective】The purpose of this program was to screen out highly virulent strains of Beauveria bassiana to Hypera postica Gyllenhal.【Method】 Ultraviolet irradiating was used to irradiate Beauveria bassiana strain for 10 min and 30 min in order to select high chitinase mutant,and then 3 stage larvae of Hypera postica underwent the virulence test with the Beauveria bassiana before and after UV mutagenesis.【Result】The results of the test indicated that corrected mortality of the stain Bb-10-12 was 100%,corrected mortality of the stain Bb-30-14 was 77.78%,and corrected mortality of wild type strain was 62.97% in 9 days later.【Conclusion】A more virulent strain Bb-10-12 of Beauveria bassiana to Hypera postica was obtained by UV mutagenesis. |
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| Keywords: | Hypera postica Gyllenhal UV mutagenizing Beauveria bassiana toxicity test |
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