4株NDV分离株F基因的克隆与序列分析

对4 株具有一定代表性的NDV(新城疫病毒)分离株的F基因进行RT PCR(反转录聚合酶链反应)扩增和序列分析,根据基因裂解位点的氨基酸序列推测,其中1 株属于弱毒株,3 株属于强毒株;核苷酸序列及其推导的氨基酸序列比较结果表明,3株强毒株与Clone30 基因核苷酸序列的同源性在83.6%~84.0%之间,与F48 E9典型NDV强毒株同源性在86.5.6%~88.3%之间,推导的氨基酸序列同源性与Clone 30 株在85.9%~87.0%之间,与F48E9典型NDV强毒株在89.1%~91.3%之间;利用MegAlign软件绘制了NDV 的系统发育进化树,结果表明, 3株分离强毒株为Ⅶ基因型,弱毒株为基因Ⅱ型。

The Cloning and Sequencing of F gene of 4 NDV Isolates

Gene fragment with F protein splitting site of 4 typical NDV isolates was amplified by RT-PCR and analyzed by sequences. Maybe one strain was low mortality virus and three strains were high mortality virus according to amino acid sequence of splitting site of gene.The result of the sequencing and comparison of sequence demonstrated that the homogeneity of nucleoside sequence among the 3 high mortality virus strains and Clone30 was 83.6%~84.0%, among typical high mortality F_(48)E_(9) strain was (86.5%)~(88.3%.)The homogeneity of amino acid sequence among the 3 high mortality virus strains and Clone30 was 85.9%~87.0%, among typical high mortality F_(48)E_(9) strain was 89.1%~91.3%. The NDV systemic evolution tree was depicted by MegAlign software and showed that the three high mortality viral strains were gene type VII and the low mortality one was gene type II.