春石斛兰类原球茎分化过程中抗氧化酶活性和可溶性蛋白的变化

以春石斛兰(Dendrobium nobileSw.)为试材,研究了类原球茎在分化芽和根系发生过程中抗氧化酶系统中多种酶活性及可溶性蛋白的变化规律。结果表明,在分化过程中抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(POD)、多酚氧化酶(PPO)和过氧化氢酶(CAT)的活性和可溶性蛋白含量均呈现先降后升的趋势;超氧化物歧化酶(SOD)活性则呈现先升后降的变化。对过氧化物酶同工酶和可溶性蛋白进行SDS-PAGE电泳发现,在分化过程中均有新谱带的产生或原有谱带的消失。

THE CHANGES OF ANTIOXIDANT ENZYME ACTIVITIES AND SOLUBLEPROTEINS DURING DIFFERENTIATION OF SHOOTS AND ROOTS FROM PROTOCORM-LIKE BODIES (PLBs) IN Dendrobium nobile in vitro

The changes of antioxidant enzyme activities and soluble proteins during the differentiation of shoots and roots from PLBs in Dendrobium nobile Sw.in vitro were systematically investigated.The results indicated that the activities of ascorbic peroxidase(APX),guaiacol peroxidase(POD),polyphenol oxidase(PPO),catalyse(CAT) and the contents of soluble proteins were decreased and then increased;and the activity of superoxide dismutase(SOD) was increased and then decreased.The formation of new bands and the disappearance of the original bands in peroxidase isozymes and soluble proteins during differentiation were also detected by using SDS-PAGE electrophoresis analysis.