| 中国樱桃半野生种对樱的茎尖培养 |
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| 引用本文： | 陈君帜,李青,孙钊.中国樱桃半野生种对樱的茎尖培养[J].园艺学报,2003,30(3):317-320. |
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| 作者姓名： | 陈君帜 李青 孙钊 |
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| 作者单位： | ( 北京林业大学园林学院，北京100083； 全国农业技术推广中心，北京100026) |
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| 摘 要： | 以中国樱桃半野生种对樱茎尖为外植体进行试管繁殖，最佳灭菌方法为二次灭菌(70％乙醇30 S→0.1％新洁尔灭15 min→0．1％升汞3 min)结合培养基添加抗生素羧苄西林二钠50 mg/L。 最佳培养条件：(1)初代培养基为F14+6-BA 0.5 mg/L+IBA0.2 mg/L+GA30.5 mg/L；(2)继代培养基为F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L，碳源采用白砂糖20 L+山梨糖醇5 g/L。同时研究表明，外植体种类、茎尖大小及抗氧化剂维生素c均影响茎尖初代培养成活率。
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| 关 键 词： | 樱桃 茎尖培养 繁殖 |
| 文章编号： | 0513-353X(2003)03-0317-02 |
| 收稿时间： | 2002-6-25 |
| 修稿时间： | 2002年6月25日 |
Studies on Shoot-tip Culture of Prunus pseudocerasus |
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| Chen Junzhi ,Li Qing ,and Sun Zhao.Studies on Shoot-tip Culture of Prunus pseudocerasus[J].Acta Horticulturae Sinica,2003,30(3):317-320. |
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| Authors: | Chen Junzhi Li Qing and Sun Zhao |
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| Institution: | Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China |
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| Abstract: | Using the shoot—tips as the explant，the tube propagation of Prunus pseudocerasus was studied. The optimal asepsis and culture condition were established by a statistical method. The explant was asepticized as follows: 70％ ethanol 30 seconds→ 0.1％ benzalkonium bromide solution 15 minutes→0.1％ HgCl2 3 minutes, besides 50 mg/L carbenicillin must be added to the media．The optimal culture condition as follows：(1)the medium for primary propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L，； (2)the medium for multiple propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L， 2％sugar and 0.5％ sorbitol．The results also showed that diferent explants． the length of shoot-tip and the application of anti-oxidant could afect the surviving rate． |
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| Keywords: | Prunus pseudocerasus Shoot-tip culture Tube propagation |
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