昆明地区奶牛临床型乳房炎病原菌分离鉴定及药敏试验

对昆明地区多个奶牛场的奶牛临床型乳房炎病原菌进行分离、鉴定,从114份乳样中共分离出细菌13种305株,经培养特性、染色镜检、生化鉴定等,鉴定出金黄色葡萄球菌63株,占20.66%;表皮葡萄球菌26株,占8.52%;无乳链球菌53株,占17.38%;停乳链球菌23株,占7.54%;乳房链球菌19株,占6.23%;乳链球菌14株,占4.59%;粪链球菌15株,占4.92%;化脓链球菌14株,占4.59%;大肠杆菌48株,占15.74%;真菌12株,占3.93%。分别对分离菌株进行17种药物的敏感性试验,除真菌外,所有菌株对庆大霉素、新霉素、环丙沙星、诺氟沙星、林可霉素、乳肿康均高度敏感,对临床常用的青霉素、链霉素、鱼腥草等具有较强的耐药性。

Isolation,identification and drug sensitivity of pathogen causingbovine clinical mastitis in Kunming area

305 strains of pathogen causing bovine clinical mastitis in Kunming area was isolated from 114 milk samples and belonged to 13 species after cultivation,staining identification and biochemical test.Among these pathogen,Staphylococcus aureus was 63 strains（20.66%）,Staphylococcus epidermidis was 26 strains（8.52%）,Streptococcus agalactiae was 53 strains（17.38%）,Streptococcus dysgalactiae was 23 strains（7.54%）,Streptococcus uberis was 19 strains（6.23%）,Streptococcus lactis was 14 strains（4.59%）,Streptococcus faecium was 15 strains（4.92%）,Streptococcus pyogenes was 14 strains（4.59%）,Escherichia coli was 48 strains（15.74%）and the fungus was 12 strains（3.93%）.Drug sensitive test showed that all bacteria except the fungus were sensitive to Gentamicin,Neomycin,Ciprofloxacin,Norfloxacin,Lincomycin etc.,and resistant to Penicilin,Streptomycin and Houttuynia cordata.