| 红茂草中异紫堇啡碱正交提取工艺的建立 |
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| 引用本文： | 赵强,王廷璞,董晓宁,沈彩霞,赵海福.红茂草中异紫堇啡碱正交提取工艺的建立[J].中兽医医药杂志,2012,31(4):5-7. |
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| 作者姓名： | 赵强 王廷璞 董晓宁 沈彩霞 赵海福 |
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| 作者单位： | 1. 甘肃工业职业技术学院化工学院,甘肃天水741025／甘肃农业大学动物医学院
2. 天水师范学院生命科学与化学学院 |
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| 基金项目： | 甘肃省中医药管理局基金项目(GZK-2010-20) |
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| 摘 要： | 采用正交工艺提取红茂草中异紫堇啡碱,在紫外光210 nm处对红茂草生物碱提取液进行检测,以异紫堇啡碱标准品作对照。结果正交提取最佳工艺参数为:乙醇浓度60%、乙醇用量200 mL、超声温度30℃、回流时间2.5 h。表明应用正交工艺对红茂草中异紫堇啡碱的提取比较合理。
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| 关 键 词： | 红茂草 异紫堇啡碱 正交设计 |
Extraction of isocodine from Dicranostigma leptodum （ Maxim ）Fedde by orthogonal design |
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| Institution: | ZHAO Qiang(Department of Chemistry Engineering,Gansu Industry Polytechnic College,Tianshui Gansu 741025,China) |
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| Abstract: | Orthogonal design was used to extract isocorydine from Dicranostigma Leptodum （Maxim.） Fedde, and the extracted liquid of alkaloids was detected by UV-VIS spectrophotometry at 210 nm using isocorydine as reference. The results demonstrated that the optimum conditions were ethanol concentration of 60%, alcohol consumption of 200 mL amt reflux time of 2.5 h at 30℃. The response surface analysis showed that this extraction process for isocorydine was reasonable. |
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| Keywords: | Dicrasitigma Leptodum（Maxim ）Fedde extraction |
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