丽格海棠高效再生体系的建立及对抗生素敏感性研究

以丽格海棠叶片和叶柄为外植体,通过离体培养分化成苗以及对卡那霉素和头孢霉素的敏感性实验,建立了农杆菌介导的稳定、高效的基因转化受体系统.结果表明:丽格海棠的最适生芽培养基为MS 6-BA0.5 rag/L NAA0.05 mg/L KT0.1 mg/L,不定芽的最适生根培养基为1/2MS IBA0.2 mg/L NAA0.15mg/L;75 mg/L卡那霉素可以完全抑制叶柄的生芽,100 mg/L卡那霉素可以完全抑制叶片的生芽,75 mg/L卡那霉素完全抑制不定芽的生根,头孢霉素浓度小于等于250 mg/L时对生芽和生根的影响不大.

Establishment of Highly Efficient Regeneration System of Rieger Begonia and Stududy on the Sensitivity to Antibiotic in Vitro

It is important to establish highly efficient regeneration system in the course of genetic transformation.The effects of hormones,Kanamycin and Cefotaxime in different concentration on shooting and rooting in Rieger Begonia in vitro were research in this study.The results showed that the optimized medium for shooting is MS 6-BA 0.5 mg/L NAA 0.05 mg/L KT 0.1 mg/L and the optimized medium for rooting is 1/2MS IBA 0.2 mg/L NAA 0.15 mg/L .Kanamycin at 75 mg/L and 100 mg/L in the medium gives effective inhibition to the regeneration of shoots from the leaf and petiole explants.In addition,Kanamycin at 75 mg/L in the medium shows obvious inhibition to the regeneration of roots from the shoots.Cefotaxime at 250 mg/L in the medium hardly shows inhibition to the refeneration of shoots from the petiole and roots from the shoots.