Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits |
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Authors: | LI Yu ZHUANG Wei-jian WANG Nai-yuan DAI Fei HONG Guo-qin XIE Na-ying LIN Jian-qin QIU Xiu-li College of Crop Science Fujian Agriculture and Forestry University Fuzhou China; |
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Institution: | 2Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;3Key Laboratory of Molecular and Cellular Biology,Fujian Province,Fuzhou 350002,China) |
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Abstract: | Using primers designed according to the published sequence of rice OsCRY1a gene,we obtained part of the gene fragment by PCR and constructed an RNA interference expression vector with it.To down-regulate the expression level of the gene or lead to the loss-of-function of the gene,the vector was then introduced into rice via Agrobacterium-mediated transformation.Based on the performance of the transgenic plants,the functions of the gene were analyzed and deduced.The results indicated that suppressing the expression of the gene retarded flowering for 16 d in rice with the plant height and grain length significantly increasing whereas other important agronomic traits observed remained unchanged apparently. |
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Keywords: | rice cryptochrome gene OsCRY1a RNA interference agronomic trait gene expression |
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