| RNAi技术及其应用 |
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| 引用本文： | 陈振东,韦晓新,吴永官.RNAi技术及其应用[J].安徽农业科学,2008,36(35). |
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| 作者姓名： | 陈振东 韦晓新 吴永官 |
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| 作者单位： | 广西农业科学院蔬菜研究中心,广西南宁,530007;广西百色国家农业科技园区,广西百色,533000 |
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| 摘 要： | RNAi主要通过双链RNA(dsRNA)被核酸酶切割成21~23 nt的干涉性小的RNA,即siRNA,由siRNA介导识别并靶向切割同源性靶mRNA分子而实现。目前RNAi技术在基因功能和基因治疗等方面的研究有了广泛的应用,RNAi技术有望成为后基因组时代基因功能分析的有力工具。
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| 关 键 词： | RNAi 作用机制 应用 |
RNAi Technology and Its Application |
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| Abstract: | RNA interference(RNAi) refers to the gene silencing phenomenon induced by double-stranded RNAs.Its realization is mainly through nuclease incising dsRNA into 21-23 nucleotide,which is a small interfering RNA(siRNA),and siRNA mediates to recognize RNAi and target to homology mRNA.At present,RNAi technology was widely used in the research of gene function and gene therapy and other fields.RNAi technology was so hopeful as to become a powerful tool for gene function analysis in the post-genome era. |
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| Keywords: | RNAi Mechanism Application |
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