| 锥虫长期超低温保藏方法的研究 |
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| 引用本文： | 白启,刘光远.锥虫长期超低温保藏方法的研究[J].畜牧兽医学报,1993,24(5):457-462. |
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| 作者姓名： | 白启 刘光远 |
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| 作者单位： | 中国农业科学院兰州兽医研究所 730046
(白启，刘光远)，中国农业科学院兰州兽医研究所 730046(周俊英) |
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| 摘 要： | 作者观测不同保护剂、稀释液、ＰＨ值、降温方法、复苏温度、冻融次数、液相气相交替以及解冻后在普通冰瓶中存放时间对伊氏锥虫浙江虫浙江虫株的感染性及致病力的影响。在此试验基础上，又对伊氏锥虫的６个不同虫株、媾疫锥虫、铡果锥虫、布氏锥虫等４个种的９个早株，进行了超低温保藏试验和长期保藏效果观察。已测定的有效保藏期伊氏锥虫达５７４－３２００天，媾疫锥虫达２８６６天，则果锥虫达７６３天，布氏锥虫７８３天。通过
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| 关 键 词： | 伊氏锥虫 保藏 低温 |
STUDIES ON THE LONG-TERM STORAGE METHOD AT ULTRA LOW-TEMPERATURE FOR TRYPANOSOMES |
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| Bai Qi,Liu Guangyuan,Zhou Junying.STUDIES ON THE LONG-TERM STORAGE METHOD AT ULTRA LOW-TEMPERATURE FOR TRYPANOSOMES[J].Acta Veterinaria et Zootechnica Sinica,1993,24(5):457-462. |
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| Authors: | Bai Qi Liu Guangyuan Zhou Junying |
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| Abstract: | The effects of different cryoprotectant, diluent, pH value, hypothermia method, anabiosis temperature, freeze-thaw times, liquid-gas exchange and the time put in general ice bottle on the infectivity and pathogenicity of Zhejiang strain of Trypanosoma evansi were observed. On the basis of these experiments, long-term storage tests with 6 strains of T. evansi and T. equiperdum, T. congolense, T. brucei at ultra low-temperature were carried out, respectively. It was demonstrated that the effective storage periods were 574-3200 days for T. evansi, 2866 days for T. equiperdum, 763 days for T. congolense, 783 days for T. brucei, respectively. Through repeated experiments, a routine storage method has been established. |
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| Keywords: | Trypanosoma evansi T equiperdum T congolense T brucei Cryo-preservation |
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