| 猪圆环病毒2型ORF2截短基因的原核表达与纯化 |
| |
| 引用本文： | 林彦星,孙彦伟,刘镇明,徐铮,鲁俊鹏,龚善中.猪圆环病毒2型ORF2截短基因的原核表达与纯化[J].动物医学进展,2006,27(12):66-70. |
| |
| 作者姓名： | 林彦星 孙彦伟 刘镇明 徐铮 鲁俊鹏 龚善中 |
| |
| 作者单位： | 1. 华南农业大学兽医学院,广东广州,510642
2. 广东省动物防疫监督总所,广东广州,510230 |
| |
| 摘 要： | 对重组原核表达载体pET-ORF2的表达条件进行优化,结果表明,最佳IPTG诱导浓度为1.0mmol/L,最佳诱导时间为5h。在最佳诱导条件下获得猪圆环病毒2型重组Cap蛋白的基础上,利用HisBind蛋白质纯化试剂盒对表达产物进行纯化,再分别用SDS-PAGE电泳和Westernblotting对纯化产物的纯化效果及特异性进行检测,结果显示表达产物纯化良好,并能被PCV-2阳性血清识别,具有良好的抗原性。
|
| 关 键 词： | 猪圆环病毒2型 重组原核表达载体 重组Cap蛋白 表达 纯化 |
| 文章编号： | 1007-5038（2006）12-0066-05 |
| 收稿时间： | 2006-09-18 |
| 修稿时间： | 2006年9月18日 |
Prokaryotic Expression of the ORF2 Truncated Gene of PCV-2 and Purification of Recombinant Protein |
| |
| LIN Yan-xing,SUN Yan-wei,LIU Zhen-ming,XU Zheng,LU Jun-peng,GONG Shan-zhong.Prokaryotic Expression of the ORF2 Truncated Gene of PCV-2 and Purification of Recombinant Protein[J].Progress In Veterinary Medicine,2006,27(12):66-70. |
| |
| Authors: | LIN Yan-xing SUN Yan-wei LIU Zhen-ming XU Zheng LU Jun-peng GONG Shan-zhong |
| |
| Institution: | 1. College of Verterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, 510640, China ; 2. Guangdong Provincial Veterinary Station of Epidemic Prevention 8,. Supervision ,Guungzhou,Guangdong,510230,China |
| |
| Abstract: | The expressive conditions of the recombinant prokaryotic expression vector pET-ORF2 was optimized in this experiment. The results demonstrated that the IPTG optimal induction concentration was 1.0 mmol/L and the best induction time was 5 h. Recombinant capsid protein of Porcine circovirus type 2 was obtained under the optimal induced condition. The expressive product was purified by His Bind Purification Kits. The purification effect and the specificity of the purified recombinant capsid protein were detected respectively by SDS-PAGE and Western blotting assay.The results showed that the recombinant Cap had been well purified and could react with the polyclonal antibody against PCV-2, and possess good antigenicity. |
| |
| Keywords: | PCV-2 recombinant prokaryotic expression vector recombinant Cap expression purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
