基因本体论在福氏志贺菌转录组研究中的应用

利用RNA-seq技术进行环丙沙星敏感和耐药的福氏志贺菌2457T株的序列测定与组装,对其碱基序列借助基因本体(Gene Ontology,GO)在生物进程、细胞组分、分子功能三方面进行注释和生物学分析。研究经过耐药诱导的转录组差异表达情况,并探讨差异表达基因在耐药性产生过程中的作用及相互关系。结果表明,通过耐药诱导有3 641个基因差异表达,其中961个GO条目注释到39个功能类别。同时发现硫酸盐、硝酸盐氧化还原相关酶系,电子/H+传导基因,膜转运蛋白,ABC外排泵家族4类耐药相关的功能基因组分。

Application of Gene Ontology Annotations in Shigella flexneri Transcriptome Study

The sequence and assemble the transcriptome of ciprofloxacin sensitive and resistant Shigella flexneri 2457T strain was conducted by RNA seq and Gene Ontology was used to annotate and analyze the base sequence in biological processes, cellular components, molecular functions. After the study of drug induced transcriptome expression differences, and the role of genes differentially expressed in the process of drug resistance and their relationship was explored. Results showed that there were 3 641 genes differentially expressed, of which 961 GO annotation entries to 39 functional categories. At the same time, drug related functional gene components were found, including sulfates and nitrates redox related enzymes, electronic / H^{+ spread genes, membrane transport proteins and ABC efflux pump family.}