丹参地上部分正丁醇萃取部位化学成分检测及其抗脑缺血研究

筛选丹参地上部分抗脑缺血的活性部位,并检测其所含化学成分,对丹参的开发和利用具有重要意义。采用D101大孔吸附树脂吸附丹参地上部分正丁醇萃取部位,将其分离纯化为多个部位。以昆明小鼠常压缺氧模型和不完全性脑缺血模型筛选有效部位;将筛选出的活性部位再用聚酰胺柱分离为不同部位,选用上述模型进行活性部位筛选;并用高效液相检测筛选的活性部位所含化学成分。结果表明:D-101大孔树脂经体积分数为50%和75%乙醇洗脱部位能显著增加小鼠常压耐缺氧时间,延长小鼠全脑缺血后的存活时间(P0.01);聚酰胺柱分离后的水洗部位可显著增加小鼠常压耐缺氧时间和小鼠全脑缺血后的存活时间(P0.01);高效液相检测聚酰胺柱分离的水洗部位含有原儿茶醛、咖啡酸、丹参素。丹参地上部分正丁醇萃取部位聚酰胺柱分离的水洗部位为有效部位,检测出有效成分为原儿茶醛、咖啡酸、丹参素。

Chemical Effective Composition and Anti-cerebral Ischemia Effect of N-butanol Extracts of Aerial Part of Salvia

It is important for Salvia utilization to screen effective composition of n-butanol extract of aerial part of Salvia against acute cerebral ischemia in mice, and to determine the active composition with HPLC. Macroporous resin D101 and polyamide column chromatography was used to divide the n-butanol extract into several portions. The active composition was screened with mice suffered from normobaric hypoxia and focal cerebral ischemia damages. At the same time the active portions from polyamide column chromatography were determined with HPLC. The results showed that the portion of 50% and 70% alcohol from D101 macroporous resin and the fraction of water elution from polyamide column chromatography increased the survival time of mice suffered from normbaric hypoxia and focal cerebral ischemia damages(P<0.01); the portion of water elution from polyamide column chromatography contain protocatechuic aldehyde, caffeic acid, tanshinol by HPLC. In conclusion, the portion of water elution from polyamide column chromatography with normal butanol extract of aerial part of Salvia was the major activity site against acute cerebral ischemic. The portion of water elution from polyamide column chromatography contain protocatechuic aldehyde, caffeic acid and tanshinol.