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Routine large-scale electrophoresis for plant breeding. An example with F1 seeds of Brussels sprouts (Brassica oleracea var.gemmifera)
Authors:L C J M Suurs
Institution:(1) Department of Plant Breeding (IvP), Agricultural University, Wageningen, the Netherlands
Abstract:Summary A routine method of large-scale electrophoresis is proposed for use in plant breeding. The method can be applied both with research and teaching and does not require sophisticated apparatus. A skilled laboratory technician can thus handle 768 samples a day. Within a series of electrophoretic investigations the individual electrophoresis can be stopped at any time, while the other investigations in the series continue.
Keywords:Brassica oleracea var  gemmifera  Brussels sprouts  F1 hybrid seed  gradient polyacrylamide gel  electrophoresis
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