Routine large-scale electrophoresis for plant breeding. An example with F1 seeds of Brussels sprouts (Brassica oleracea var.gemmifera) |
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Authors: | L C J M Suurs |
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Institution: | (1) Department of Plant Breeding (IvP), Agricultural University, Wageningen, the Netherlands |
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Abstract: | Summary A routine method of large-scale electrophoresis is proposed for use in plant breeding. The method can be applied both with research and teaching and does not require sophisticated apparatus. A skilled laboratory technician can thus handle 768 samples a day. Within a series of electrophoretic investigations the individual electrophoresis can be stopped at any time, while the other investigations in the series continue. |
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Keywords: | Brassica oleracea var gemmifera Brussels sprouts F1 hybrid seed gradient polyacrylamide gel electrophoresis |
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