| 葡萄糖在牛体外受精及胚胎发育中的影响 |
| |
| 引用本文： | 彭礼繁,罗光彬,李东全,陈自洪,石德顺.葡萄糖在牛体外受精及胚胎发育中的影响[J].中国草食动物,2008,28(5). |
| |
| 作者姓名： | 彭礼繁 罗光彬 李东全 陈自洪 石德顺 |
| |
| 作者单位： | 1. 沈阳农业大学动物胚胎工程实验室,辽宁,110161
2. 广西大学动物繁殖研究所 |
| |
| 摘 要： | 在牛精子获能液中添加不同浓度的葡萄糖,应用体外培养技术对牛精子体外获能及早期胚胎发育进行研究,通过观察顶体反应、超激活、活率和早期胚胎发育率,筛选出获能液中最适葡萄糖浓度及其有利于牛早期胚胎发育的最佳浓度.结果表明:葡萄糖是精子获能和维持超激活运动的主要能源物质,其代谢过程中产生的活性氧在牛精子体外获能、受精过程中起重要作用,高浓度(超过9.15 mM)葡萄糖有利于获能的完成;但是对早期胚胎发育不利,对早期胚胎发育来说其最适添加量为6.10 mM,此时的囊胚率最高.
|
| 关 键 词： | 葡萄糖 牛 体外受精 胚胎发育 |
Effects of Glucose on Bovine in Vitro Fertilization and Embryo Development |
| |
| Peng Li-fan,Luo Guang-bin,Li Dong-quan,et al.Effects of Glucose on Bovine in Vitro Fertilization and Embryo Development[J].China Herbivores,2008,28(5). |
| |
| Authors: | Peng Li-fan Luo Guang-bin Li Dong-quan |
| |
| Institution: | Animal Embryo Engineering Laboratory of Shenyang Agricultural University;Liaoning 110161 |
| |
| Abstract: | Through the alteration of glucose in the capacitation medium and the use of culture in vitro,the bovine sperm capacitation and early embryo development were studied in this research.Through the observation of acrosome reaction,hyperpolarization,motility and early embryo development,the optimal concentration of glucose for the capacitation medium and bovine early embryo development were selected.The results showed that glucose played an important role in the process of bovine sperm capacitation and hyperpola... |
| |
| Keywords: | glucose bovine in vitro fertilization embryo development |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
