| 茶树Bt和Cpti双价抗虫基因转化研究（简报） |
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| 引用本文： | 成杨,赵洋,杨培迪,杨阳,刘振.茶树Bt和Cpti双价抗虫基因转化研究（简报）[J].茶叶通讯,2014,41(4):41-43. |
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| 作者姓名： | 成杨 赵洋 杨培迪 杨阳 刘振 |
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| 作者单位： | 湖南省茶叶研究所,湖南长沙,410125 |
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| 摘 要： | 利用转基因获得抗虫茶树品种是防治茶树害虫的重要途径。将含有Bt和Cpti双价抗虫基因的质粒转入感受态E.coitl DH5α菌株并进行培养、提取和扩增后的质粒,注入茶树花期的子房内,种植接种后的茶籽,并对获得的植株进行检测。
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| 关 键 词： | 茶树 花粉管通道法 Bt Cpti |
Transform of Bt and Cpti into tea plant (Camellia sinesis) |
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| CHENG Yang,ZHAO Yang,YANG Pei-di,YANG Yang,LIU Zhen.Transform of Bt and Cpti into tea plant (Camellia sinesis)[J].Tea Communication,2014,41(4):41-43. |
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| Authors: | CHENG Yang ZHAO Yang YANG Pei-di YANG Yang LIU Zhen |
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| Institution: | ( Hunan Tea Research Institute, Changsha 410125, China) |
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| Abstract: | To obtain pest resistence by genetically modification is one of the important techinique in tea pest control. Plasmid with Bt and Cpti was introduced into competent E.coli DH5a strain, and cultured. The amplified plasmid was extracted and injected into florescene tea ovary. The inoculated tea seed was planted, and the growing up tea tree was tested. |
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| Keywords: | Tea tree Pollen tube pathway method Bt Cpti |
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