| ISSR及其在果树上的应用 |
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| 引用本文： | 张青林,罗正荣. ISSR及其在果树上的应用[J]. 果树学报, 2004, 21(1): 54-58 |
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| 作者姓名： | 张青林 罗正荣 |
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| 作者单位： | 华中农业大学园艺林学学院,湖北,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助项目(30270925) |
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| 摘 要： | ISSR是一种基于微卫星序列发展起来的新的分子标记,具有简便、稳定、DNA多态性高等优点。ISSR标记呈孟德尔式遗传,大部分ISSR标记为显性标记。综合有关文献就ISSR的原理、操作及其在果树种质鉴定、遗传多样性检测、亲缘关系分析和遗传图谱构建等方面的应用和进展进行简要的阐述。
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| 关 键 词： | ISSR 种质鉴定 遗传多样性检测 亲缘关系分析 遗传图谱构建 果树 |
| 文章编号： | 1009-9980(2004)01-054-05 |
| 修稿时间： | 2003-05-20 |
ISSR Technology and Its Applications in Fruit Trees |
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| Abstract: | Inter-simple sequence repeat(ISSR)is a microsatellite-based technique being used for genetic and breeding studies as a new molecular marker.ISSR markers are a quick,simple and reliable method and may also generate greater DNA polymorphism.ISSR markers are inherited and segr egated in Mendelian fashion and most of which behave as dominant markers.This paper emphasizes on the principle and procedures of ISSR and its application to germplasm identification,ge-netic diversity detection,phylogenetic analysis, and genetic map construction in fruit trees. |
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| Keywords: | Inter-simple sequence repeat(ISSR) Germplasm identification Genetic diversity detection Phylogene tic analysis Genetic map construction Fruit trees |
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