水杨酸和一氧化氮对姜黄生长及次生代谢产物的影响

以姜黄(Curcuma longa L.)组培苗为试材,在继代培养基中添加不同浓度水杨酸(SA)和硝普钠(SNP,NO供体),研究SA和NO对姜黄生长、次生代谢和姜黄素类化合物含量的影响。试验结果表明:SA和NO均能调节姜黄的生长,促进姜黄次生代谢产物的生成;SA 10μmol·L-1或SNP 200μmol·L-1处理时,姜黄叶片叶绿素含量、类胡萝卜素含量和可溶性糖含量均达到最大值,姜黄生长处于最佳状态。SA和NO均能激活苯丙氨酸解氨酶(PAL)、肉桂酸–4–羟化酶(C4H)和4–香豆酸:辅酶A连接酶(4CL)活性,促进姜黄次生代谢途径中咖啡酸、对香豆酸、阿魏酸和肉桂酸的生成,提高双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量;因此,SA 10μmol·L-1或SNP 200μmol·L-1最有利于姜黄生长和次生代谢产物的合成。SA和NO对姜黄次生代谢生理调控具有浓度效应。

Effects of Salicylic Acid and Nitric Oxide on the Growth and Accumulation of Secondary Metabolites in Curcuma longa

The effects of nitric oxide（NO）and salicylic acid（SA）on the growth and physiological characteristics，key enzymatic activity of secondary metabolic pathways，the content of organic acid and curcuminoids of in vitro Curcuma longa plantlets were studied. And the medium was supplemented with sodium nitroprusside（SNP）（0，50，100，200，400 and 800 μmol ? L-1） as a donor of NO and salicylic acid（0，5，10，20，40 and 80 μmol ? L-1）. The results showed that NO and SA can adjust the growth and physiological characteristics and promoted the accumulation of secondary metabolites of Curcuma longa in some degree. It raised significantly the contents of chlorophyll，carotenoid，soluble sugar and protein in leaves of Curcuma longa，and activated the activities of phenylalanin ammonia-lyase（PAL），cinnamic acid-4-hydroxylase（C4H）and 4-coumaric acid：CoA ligase（4CL），promoted the accumulation ofcinnamic acid，p-coumaric acid，coffee acid and ferulic acid，and then improved the content of curcumin and demethoxycurcumin，bisdemethoxycurcumin with 200 μmol ? L-1SNP and 10 μmol ? L-1 SA. Therefore，200 μmol ? L-1SNP and 10 μmol ? L-1 SA were conducive to the growth and accumulation of secondary metabolites in Curcuma longa. It suggested that NO and SA have dual nature on secondary metabolites physiological Abstract. characteristics of Curcuma longa.